EK2392

Mouse Total HIF-1α/HIF1A ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：HIF1A, HIF-1α, MOP1
种属：小鼠 (Mouse)
样本类型：细胞裂解产物及其他生物学样本
检测样本体积：10 μL
灵敏度：0.46 pg/mL
检测范围：23.44 pg/mL - 1500 pg/mL
回收率：81% - 117%

在售SKU:70-EK2392-48, 70-EK2392-96, EK2392

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文章目录[隐藏]

HIF1A 分子靶点信息概述
HIF1A 分子靶点综述
HIF1A, hypoxia inducible factor 1 subunit alpha 分子靶点种属信息
- 小鼠 Mouse Hif1a 分子靶点信息
- 小鼠 Mouse Hif1a 靶点分子功能（预测）

商品名	Mouse Total HIF-1α/HIF1A ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法	双抗夹心法
精密度	板内变异系数：4.5% - 6.3%；板间变异系数：7.1% - 7.5%
样本类型	细胞裂解产物及其他生物学样本
检测样本体积	10 μL
灵敏度	0.46 pg/mL
检测范围	23.44 pg/mL - 1500 pg/mL
回收率	81% - 117%
平均回收率	0.97
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被鼠抗人Total HIF-1α/HIF1A捕获抗体的96孔聚苯乙烯酶标板 Mouse Total HIF-1α/HIF1A冻干标准品 Mouse Total HIF-1α/HIF1A检测抗体 Streptavidin-HRP 检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜标准品稀释液

检测原理：本试本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse Total HIF-1α/HIF1A ELISA Kit 检测试剂盒（酶联免疫吸附法） - 标准曲线 — 标准曲线

HIF1A 分子靶点信息概述

分子名：HIF1A, hypoxia inducible factor 1 subunit alpha
基因家族：Basic helix-loop-helix proteins; PAS domain containing
别名：MOP1; HIF-1alpha; PASD8; HIF1; bHLHe78
全称：hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); PAS domain-containing protein 8; Class E basic helix-loop-helix protein 78; Member of PAS protein 1

HIF1A 分子靶点综述

低氧诱导转录因子1α(HIF-1α/HIF1A)是转录因子异二聚体HIF-1α/β的调控成员。作为对缺氧的适应性反应的主要转录调节因子，HIF-1α在低氧条件下，与许多基因启动子中的缺氧反应元件(HREs)结合，激活40多个基因的转录，包括促红细胞生成素、葡萄糖转运蛋白、糖酵解酶、血管内皮生长因子、HILPDA和其他其蛋白产物增加氧输送或促进代谢对低氧适应的基因。HIF-1α还在胚胎血管生成、肿瘤血管生成和缺血性疾病的病理生理中起重要作用以及参与缺氧时神经元内线粒体的轴突分布和运输。
HIF-1α普遍存在于人和哺乳动物细胞内，常氧下(21% O₂)也有表达，但合成的HIF-1α蛋白很快被细胞内氧依赖性泛素蛋白酶降解途径所降解，只有在缺氧条件下HIF-1α才可稳定表达。

HIF1A, hypoxia inducible factor 1 subunit alpha 分子靶点种属信息

小鼠 Mouse

小鼠 Mouse Hif1a 分子靶点信息

分子名：Hif1a, hypoxia inducible factor 1, alpha subunit
别称：
- AA959795
- bHLHe78
- expressed sequence AA959795
- HIF-1alpha
- HIF1alpha
- MOP1
基因序列：NCBI_Gene: 15251
蛋白序列：

小鼠 Mouse Hif1a 靶点分子功能（预测）

Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; E-box binding activity; and histone deacetylase binding activity. Involved in several processes, including connective tissue replacement involved in inflammatory response wound healing; positive regulation of autophagy of mitochondrion; and positive regulation of miRNA transcription. Acts upstream of or within several processes, including circulatory system development; negative regulation of apoptotic process; and regulation of nucleobase-containing compound metabolic process. Located in euchromatin; motile cilium; and nuclear speck. Is expressed in several structures, including central nervous system; genitourinary system; hemolymphoid system; lung; and sensory organ. Used to study glycogen storage disease V and glycogen storage disease VII. Human ortholog(s) of this gene implicated in carcinoma (multiple); diabetic retinopathy; myocardial infarction; prostate cancer; and type 2 diabetes mellitus. Orthologous to human HIF1A (hypoxia inducible factor 1 subunit alpha).