EK1392

Human Total HIF-1α/HIF1A ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:HIF1A, HIF-1α, MOP1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL (稀释液)
  • 灵敏度:1.55 pg/mL
  • 检测范围:23.44 pg/mL - 1500 pg/mL
  • 回收率:83% - 123%

在售SKU:70-EK1392-48, 70-EK1392-96

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描述

商品名

Human Total HIF-1α/HIF1A ELISA Kit 人总低氧诱导转录因子1α(HIF-1α/HIF1A)ELISA试剂盒

检测方法

双抗夹心法

精密度

板内变异系数:5.3% - 7.6%;板间变异系数:5.7% - 6.9%

样本类型

血清、血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL (稀释液)

灵敏度

1.55 pg/mL

检测范围

23.44 pg/mL - 1500 pg/mL

回收率

83% - 123%

平均回收率

1.00

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗人HIF-1α抗体的96孔聚苯乙烯酶标板
  • Human HIF-1α冻干标准品
  • Human HIF-1α检测抗体
  • Streptavidin-HRP
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜
  • 标准品稀释液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人 HIF-1α 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的 HIF-1α 与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物 TMB,避光显色。 颜色反应的深浅与样本中 HIF-1α 的浓度成正比。加入终止液终止反应,在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Human Total HIF-1α/HIF1A ELISA Kit 检测试剂盒(酶联免疫吸附法) - 标准曲线
标准曲线

HIF1A 分子靶点信息概述

  • 分子名:HIF1A, hypoxia inducible factor 1 subunit alpha
  • 基因家族:Basic helix-loop-helix proteins; PAS domain containing
  • 别名:MOP1; HIF-1alpha; PASD8; HIF1; bHLHe78
  • 全称:hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); PAS domain-containing protein 8; Class E basic helix-loop-helix protein 78; Member of PAS protein 1

HIF1A 分子靶点综述

低氧诱导转录因子1α(HIF-1α/HIF1A)是转录因子异二聚体HIF-1α/β的调控成员。作为对缺氧的适应性反应的主要转录调节因子,HIF-1α在低氧条件下,与许多基因启动子中的缺氧反应元件(HREs)结合,激活40多个基因的转录,包括促红细胞生成素、葡萄糖转运蛋白、糖酵解酶、血管内皮生长因子、HILPDA和其他其蛋白产物增加氧输送或促进代谢对低氧适应的基因。HIF-1α还在胚胎血管生成、肿瘤血管生成和缺血性疾病的病理生理中起重要作用以及参与缺氧时神经元内线粒体的轴突分布和运输。
HIF-1α普遍存在于人和哺乳动物细胞内,常氧下(21% O2)也有表达,但合成的HIF-1α蛋白很快被细胞内氧依赖性泛素蛋白酶降解途径所降解,只有在缺氧条件下HIF-1α才可稳定表达。

HIF1A, hypoxia inducible factor 1 subunit alpha 分子靶点种属信息

人 Human

人 Human HIF1A 分子靶点信息

  • 分子名:HIF1A, hypoxia inducible factor 1 subunit alpha
  • 别称:
    • ARNT interacting protein
    • ARNT-interacting protein
    • basic-helix-loop-helix-PAS protein MOP1
    • bHLHe78
    • class E basic helix-loop-helix protein 78
    • HIF-1-alpha
    • HIF-1A
    • HIF-1alpha
    • HIF1
    • HIF1-ALPHA
    • hypoxia inducible factor 1 alpha subunit
    • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
    • hypoxia-inducible factor 1 alpha isoform I.3
    • hypoxia-inducible factor 1-alpha
    • hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
    • hypoxia-inducible factor1alpha
    • member of PAS protein 1
    • member of PAS superfamily 1
    • MOP1
    • PAS domain-containing protein 8
    • PAS domain-containing protein 8", "Class E basic helix-loop-helix protein 78", "Member of PAS protein 1
    • PASD8
  • 基因序列:NCBI_Gene: 3091
  • 蛋白序列:UniProtKB: Q16665

人 Human HIF1A靶点分子功能(预测)

Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; Hsp90 protein binding activity; and enzyme binding activity. Contributes to DNA-binding transcription factor activity, RNA polymerase II-specific and sequence-specific DNA binding activity. Involved in several processes, including oxygen homeostasis; regulation of gene expression; and vascular endothelial growth factor production. Acts upstream of or within regulation of transcription, DNA-templated and response to hypoxia. Located in chromatin; cytosol; and nuclear body. Part of RNA polymerase II transcription regulator complex. Implicated in lung non-small cell carcinoma; myocardial infarction; prostate cancer; and type 2 diabetes mellitus. Biomarker of several diseases, including arthritis (multiple); gastrointestinal system cancer (multiple); invasive ductal carcinoma; kidney cancer (multiple); and morbid obesity.

检测步骤


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