EK1C01

Human Pro-Collagen I α1 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

  • 分子靶点:COL1A1, Pro-Collagen I α1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL (稀释液)
  • 灵敏度:0.67 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:72% - 112%

在售SKU:70-EK1C01-48, 70-EK1C01-96, 人, I, 胶原蛋白, I型, 前胶原蛋白, 前胶原蛋白I, 胶原蛋白 I, 人I型前胶原蛋白 α1

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描述

商品名

Human Pro-Collagen Iα1 ELISA Kit 人I型前胶原蛋白(Human Pro-Collagen Iα1)ELISA试剂盒

检测方法

双抗夹心法

精密度

板内变异系数:2.3% - 3.8%;板间变异系数:1.0% - 1.2%

样本类型

血清、血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL (稀释液)

灵敏度

0.67 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

72% - 112%

平均回收率

0.90

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被小鼠抗人Pro-Collagen Iα1捕获抗体的96孔聚苯乙烯酶标板
  • Human Pro-Collagen Iα1冻干标准品
  • Human Pro-Collagen Iα1检测抗体
  • Streptavidin-HRP
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜
  • 标准品稀释液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Pro-COL I A1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的Pro-COL I A1与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Pro-COL I A1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Pro-Collagen Iα1 ELISA KIT 人I型前胶原蛋白(Human Pro-Collagen Iα1)ELISA试剂盒 - 标准曲线
标准曲线

COL1A1 分子靶点信息概述

  • 分子名:COL1A1, collagen type I alpha 1 chain
  • 基因家族:Collagens
  • 别名:collagen, type I, alpha 1; OI4

COL1A1 分子靶点综述

I 型胶原蛋白是皮肤、骨骼和肌腱等结缔组织中含量最丰富的结构蛋白。它是一种合成的前胶原分子,其特征为 300 nm 的三重螺旋结构域,两侧是球状的 N 端和 c 端前肽。三螺旋结构域包含 Gly-Xaa-Yaa 三联体,其中 Xaa 和 Yaa 通常分别是脯氨酸和羟脯氨酸。前胶原蛋白 N 和 C 蛋白酶活性去除非螺旋前肽,因此成熟的三螺旋可以自组装成胶原原纤维,为组织提供拉伸强度。

COL1A1, collagen type I alpha 1 chain 分子靶点种属信息

人 Human

人 Human COL1A1 分子靶点信息

  • 分子名:COL1A1, collagen type I alpha 1 chain
  • 别称:
    • alpha-1 type I collagen
    • alpha1(I) procollagen
    • CAFYD
    • collagen alpha 1 chain type I
    • collagen alpha-1(I) chain
    • collagen alpha-1(I) chain preproprotein
    • collagen of skin, tendon and bone, alpha-1 chain
    • collagen type I alpha 1
    • collagen, type I, alpha 1
    • EDSARTH1
    • EDSC
    • OI1
    • OI2
    • OI3
    • OI4
    • pro-alpha-1 collagen type 1
    • type I proalpha 1
    • type I procollagen alpha 1 chain
  • 基因序列:NCBI_Gene: 1277
  • 蛋白序列:UniProtKB: P02452

人 Human COL1A1靶点分子功能(预测)

Enables identical protein binding activity; platelet-derived growth factor binding activity; and protease binding activity. Involved in several processes, including collagen biosynthetic process; collagen fibril organization; and skin morphogenesis. Acts upstream of or within skeletal system development. Located in extracellular space. Part of collagen type I trimer. Colocalizes with collagen-containing extracellular matrix. Implicated in several diseases, including Ehlers-Danlos syndrome arthrochalasia type 1; aggressive periodontitis; bone disease (multiple); cutaneous leishmaniasis; and dentinogenesis imperfecta. Biomarker of several diseases, including alcoholic hepatitis; autoimmune disease (multiple); chronic obstructive pulmonary disease; end stage renal disease; and lupus nephritis.

检测步骤


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