EK1C01

Human Pro-Collagen I α1 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：COL1A1, Pro-Collagen I α1
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL (稀释液)
灵敏度：0.67 pg/mL
检测范围：15.63 pg/mL - 1000 pg/mL
回收率：72% - 112%

在售SKU:70-EK1C01-48, 70-EK1C01-96, 人, I, 胶原蛋白, I型, 前胶原蛋白, 前胶原蛋白I, 胶原蛋白 I, 人I型前胶原蛋白 α1

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库存号

描述

文章目录[隐藏]

COL1A1 分子靶点信息概述
COL1A1 分子靶点综述
COL1A1, collagen type I alpha 1 chain 分子靶点种属信息
- 人 Human COL1A1 分子靶点信息
- 人 Human COL1A1靶点分子功能（预测）

商品名	Human Pro-Collagen Iα1 ELISA Kit 人I型前胶原蛋白(Human Pro-Collagen Iα1)ELISA试剂盒
检测方法	双抗夹心法
精密度	板内变异系数：2.3% - 3.8%；板间变异系数：1.0% - 1.2%
样本类型	血清、血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL (稀释液)
灵敏度	0.67 pg/mL
检测范围	15.63 pg/mL - 1000 pg/mL
回收率	72% - 112%
平均回收率	0.90
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被小鼠抗人Pro-Collagen Iα1捕获抗体的96孔聚苯乙烯酶标板 Human Pro-Collagen Iα1冻干标准品 Human Pro-Collagen Iα1检测抗体 Streptavidin-HRP 检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜标准品稀释液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Pro-COL I A1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的Pro-COL I A1与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Pro-COL I A1的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Pro-Collagen Iα1 ELISA KIT 人I型前胶原蛋白(Human Pro-Collagen Iα1)ELISA试剂盒 - 标准曲线 — 标准曲线

COL1A1 分子靶点信息概述

分子名：COL1A1, collagen type I alpha 1 chain
基因家族：Collagens
别名：collagen, type I, alpha 1; OI4

COL1A1 分子靶点综述

I 型胶原蛋白是皮肤、骨骼和肌腱等结缔组织中含量最丰富的结构蛋白。它是一种合成的前胶原分子，其特征为 300 nm 的三重螺旋结构域，两侧是球状的 N 端和 c 端前肽。三螺旋结构域包含 Gly-Xaa-Yaa 三联体，其中 Xaa 和 Yaa 通常分别是脯氨酸和羟脯氨酸。前胶原蛋白 N 和 C 蛋白酶活性去除非螺旋前肽，因此成熟的三螺旋可以自组装成胶原原纤维，为组织提供拉伸强度。

COL1A1, collagen type I alpha 1 chain 分子靶点种属信息

人 Human

人 Human COL1A1 分子靶点信息

分子名：COL1A1, collagen type I alpha 1 chain
别称：
- alpha-1 type I collagen
- alpha1(I) procollagen
- CAFYD
- collagen alpha 1 chain type I
- collagen alpha-1(I) chain
- collagen alpha-1(I) chain preproprotein
- collagen of skin, tendon and bone, alpha-1 chain
- collagen type I alpha 1
- collagen, type I, alpha 1
- EDSARTH1
- EDSC
- OI1
- OI2
- OI3
- OI4
- pro-alpha-1 collagen type 1
- type I proalpha 1
- type I procollagen alpha 1 chain
基因序列：NCBI_Gene: 1277
蛋白序列：UniProtKB: P02452

人 Human COL1A1靶点分子功能（预测）

Enables identical protein binding activity; platelet-derived growth factor binding activity; and protease binding activity. Involved in several processes, including collagen biosynthetic process; collagen fibril organization; and skin morphogenesis. Acts upstream of or within skeletal system development. Located in extracellular space. Part of collagen type I trimer. Colocalizes with collagen-containing extracellular matrix. Implicated in several diseases, including Ehlers-Danlos syndrome arthrochalasia type 1; aggressive periodontitis; bone disease (multiple); cutaneous leishmaniasis; and dentinogenesis imperfecta. Biomarker of several diseases, including alcoholic hepatitis; autoimmune disease (multiple); chronic obstructive pulmonary disease; end stage renal disease; and lupus nephritis.