EK102

Human IL-2 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥10,800.00

  • 分子靶点:IL2, IL-2
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:0.36 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:86% - 97%

在售SKU:70-EK102-48, 70-EK102-96, 人白介素2, 白介素2, 人, 白介素, 2

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描述

商品名

Human IL-2 ELISA Kit (人白介素2 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.5% - 3.6%;板间变异系数:3.2% - 7.0%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

0.36 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

86% - 97%

平均回收率

0.91

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-2单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-2冻干标准品
  • IL-2检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-2与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-2的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-2 ELISA Kit (人白介素2 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL2, IL-2 靶点信息

IL2 分子靶点信息概述

  • 分子名:IL2, interleukin 2
  • 基因家族:Interleukins
  • 别名:IL-2; TCGF
  • 全称:T cell growth factor

IL2 分子靶点综述

白细胞介素2(IL-2)是15kDa的糖蛋白,在人类中,由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子,调控对具有免疫功能的白血球(白细胞,通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答,区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能,其受体由3条链组成,分别为α链(CD25)、β链(CD122)和γ链(CD132)。监测血清中的IL-2含量为多种病理状态提供更详细的视角,包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。

人 Human IL2 分子靶点信息

  • 分子名:IL2, interleukin 2
  • 别称:
    • aldesleukin
    • IL-2
    • interleukin-2
    • involved in regulation of T-cell clonal expansion
    • lymphokine
    • T cell growth factor
    • TCGF
  • 基因序列:NCBI_Gene: 3558
  • 蛋白序列:UniProtKB: P60568

人 Human IL2靶点分子功能(预测)

Enables cytokine activity and interleukin-2 receptor binding activity. Involved in leukocyte activation involved in immune response; positive regulation of interleukin-17 production; and positive regulation of tyrosine phosphorylation of STAT protein. Acts upstream of or within negative regulation of B cell apoptotic process; positive regulation of B cell proliferation; and positive regulation of activated T cell proliferation. Predicted to be located in extracellular space. Implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Biomarker of several diseases, including Zellweger syndrome; aggressive periodontitis; anogenital venereal wart; autoimmune disease (multiple); and uveitis (multiple).

操作步骤


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引用文献


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  3. Increased Antitumor Activities of Glypican-3-Specific Chimeric Antigen Receptor-Modified T Cells by Coexpression of a Soluble Pd1-Ch3 Fusion Protein 

  4. Recombination of a Dual-Car-Modified T Lymphocyte to Accurately Eliminate Pancreatic Malignancy 

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