EK2135

Mouse osteopontin/OPN ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：SPP1, OPN, Osteopontin
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：10 μL
灵敏度：0.05 pg/mL
检测范围：15.63 pg/mL - 1000 pg/mL
回收率：89% - 106%

在售SKU:70-EK2135/2-48, 70-EK2135/2-96

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库存号

描述

文章目录[隐藏]

分子信息
SPP1 分子靶点信息概述
SPP1 分子靶点综述
- 小鼠 Mouse Spp1 分子靶点信息
- 小鼠 Mouse Spp1 靶点分子功能（预测）

商品名	Mouse osteopontin/OPN ELISA Kit (小鼠骨桥蛋白 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：1.8% - 3.4%；板间变异系数：2.1% - 4.7%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	10 μL
灵敏度	0.05 pg/mL
检测范围	15.63 pg/mL - 1000 pg/mL
回收率	89% - 106%
平均回收率	0.98
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗OPN单克隆抗体的96孔聚苯乙烯酶标板小鼠OPN冻干标准品 OPN检测抗体辣根过氧化物酶标记的链霉亲和素检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠 OPN 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的 OPN 与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物 TMB，避光显色。颜色反应的深浅与样本中 OPN 的浓度成正比。加入终止液终止反应，在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Mouse osteopontin/OPN ELISA Kit (小鼠骨桥蛋白 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 SPP1, OPN, Osteopontin 靶点信息

SPP1 分子靶点信息概述

分子名：SPP1, secreted phosphoprotein 1
基因家族：Receptor ligands; SIBLING family
别名：BSPI; ETA-1
曾用名：BNSP; OPN
全称：early T-lymphocyte activation 1; osteopontin; bone sialoprotein I

SPP1 分子靶点综述

骨桥蛋白(OPN)，又名BSP-1、ETA-1、SPP-1或Ric，在人类中由SPP1基因编码，主要在骨骼中表达。OPN基因的调节机制还未完全阐明，不同细胞类型的调节机理是不同的。OPN在生物矿化、骨重塑、免疫功能、趋化、细胞活化和凋亡上有作用，同样与心肌细胞凋亡和心肌功能紊乱的增加有关。OPN与许多生理和病理过程包括伤口愈合、骨转换、肿瘤发生、炎症、缺血和免疫应答有关。调控血浆(或局部)的OPN水平对治疗自身免疫性疾病、癌症转移、骨和牙齿的矿化、骨质疏松症和一些应激形式有帮助。

小鼠 Mouse Spp1 分子靶点信息

分子名：Spp1, secreted phosphoprotein 1
别称：
- 2ar
- 44kDa bone phosphoprotein
- AA960535
- activation protein lymphocyte 1
- AI790405
- Apl-1
- BNSP
- bone sialoprotein 1
- early T lymphocyte activation
- Eta
- ETA-1
- expressed sequence AA960535
- expressed sequence AI790405
- minopontin
- OP
- Opn
- Opnl
- osteopontin
- osteopontin-like protein
- Ric
- rickettsia tsutsugamushi resistance
- Spp-1
基因序列：NCBI_Gene: 20750
蛋白序列：

小鼠 Mouse Spp1 靶点分子功能（预测）

Enables extracellular matrix binding activity. Acts upstream of or within several processes, including cellular ion homeostasis; cellular response to leukemia inhibitory factor; and neutrophil chemotaxis. Located in apical part of cell and cytoplasm. Is expressed in several structures, including alimentary system; brain; metanephros; reproductive system; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; coronary artery disease (multiple); disease of cellular proliferation (multiple); and hepatitis. Orthologous to human SPP1 (secreted phosphoprotein 1).