EK183EG

EasyGo!™ Human VEGF One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥2,190.00 – ¥3,650.00

说明书

分子靶点：VEGFA, VEGF, VPF
种属：人 (Human)
样本类型：血清，血浆，DMEM细胞培养上清
检测样本体积：血清/血浆：20 μL；DMEM细胞培养上清：100 μL
灵敏度：3.03 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：85% - 112%

在售SKU:70-EK183EG-48, 70-EK183EG-96, EK183EG, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒, 人血管内皮生长因子, 血管内皮生长因子, VEGF

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库存号

描述

文章目录[隐藏]

分子信息
VEGFA 分子靶点信息概述
VEGFA 分子靶点综述
- 人 Human VEGFA 分子靶点信息
- 人 Human VEGFA靶点分子功能（预测）

商品名	EasyGo!™ Human VEGF One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法	一步法
精密度	板内变异系数：4.1% - 6.4%；板间变异系数：2.1% - 2.6%
样本类型	血清，血浆，DMEM细胞培养上清
检测样本体积	血清/血浆：20 μL；DMEM细胞培养上清：100 μL
灵敏度	3.03 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	85% - 112%
平均回收率	0.94
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	含微球的预包被酶标板标准品标准品稀释液显色底物终止液 20×洗液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上，酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

EasyGo!™ Human VEGF One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）- 标准曲线 — 标准曲线

分子信息

概述人 VEGFA, VEGF, VPF 靶点信息

VEGFA 分子靶点信息概述

分子名：VEGFA, vascular endothelial growth factor A
基因家族：VEGF family
别名：VEGF-A; VPF
曾用名：VEGF
全称：vascular endothelial growth factor

VEGFA 分子靶点综述

血管内皮生长因子(VEGF)是生长因子的亚家族，具体来说，属于胱氨酸结生长因子中的血小板衍生生长因子(PDGF)家族。它们是重要的信号转导蛋白，参与血管发生(胚胎循环系统从头形成)和血管生成(从已存在的脉管系统生长形成血管)。VEGF可刺激血管发生和血管生成。当血液循环不足时，可参与回复组织供氧系统。VEGF最主要的作用是血管生成。在胚胎形成期，VEGF调控内皮细胞的增殖、迁移和存活，从而调控血管密度和大小，但无法决定血管的类型。在支气管哮喘和糖尿病患者的血清中含有高浓度VEGF。当VEGF过表达时会引起疾病。供血不足时，实体肿瘤的大小受限；而表达VEGF的肿瘤则会不断生长并发生转移。VEGF过表达会导致眼睛视网膜和身体其他部分的血管性疾病。贝伐珠单抗和雷珠单抗可抑制VEGF，并控制或减缓疾病进程。

人 Human VEGFA 分子靶点信息

分子名：VEGFA, vascular endothelial growth factor A
别称：
- MGC70609
- MVCD1
- vascular endothelial growth factor
- vascular endothelial growth factor A121
- vascular endothelial growth factor A165
- vascular permeability factor
- VEGF
- VEGF-A
- VPF
基因序列：NCBI_Gene: 7422
蛋白序列：UniProtKB: P15692

人 Human VEGFA靶点分子功能（预测）

Enables several functions, including fibronectin binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell migration; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including branching involved in blood vessel morphogenesis; positive regulation of endothelial cell proliferation; and regulation of nitric oxide mediated signal transduction. Located in several cellular components, including adherens junction; cell surface; and secretory granule. Implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); inflammatory bowel disease (multiple); and respiratory system cancer (multiple).