EK183EG

EasyGo!™ Human VEGF One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥2,190.00¥3,650.00

  • 分子靶点:VEGFA, VEGF, VPF
  • 种属:人 (Human)
  • 样本类型:血清,血浆,DMEM细胞培养上清
  • 检测样本体积:血清/血浆:20 μL;DMEM细胞培养上清:100 μL
  • 灵敏度:3.03 pg/mL
  • 检测范围:31.25 pg/mL - 2000 pg/mL
  • 回收率:85% - 112%

在售SKU:70-EK183EG-48, 70-EK183EG-96, EK183EG, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒, 人血管内皮生长因子, 血管内皮生长因子, VEGF

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描述

商品名

EasyGo!™ Human VEGF One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

一步法

精密度

板内变异系数:4.1% - 6.4%;板间变异系数:2.1% - 2.6%

样本类型

血清,血浆,DMEM细胞培养上清

检测样本体积

血清/血浆:20 μL;DMEM细胞培养上清:100 μL

灵敏度

3.03 pg/mL

检测范围

31.25 pg/mL - 2000 pg/mL

回收率

85% - 112%

平均回收率

0.94

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 含微球的预包被酶标板
  • 标准品
  • 标准品稀释液
  • 显色底物
  • 终止液
  • 20×洗液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

EasyGo!™ Human VEGF One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)- 标准曲线
标准曲线

分子信息

概述人 VEGFA, VEGF, VPF 靶点信息

VEGFA 分子靶点信息概述

  • 分子名:VEGFA, vascular endothelial growth factor A
  • 基因家族:VEGF family
  • 别名:VEGF-A; VPF
  • 曾用名:VEGF
  • 全称:vascular endothelial growth factor

VEGFA 分子靶点综述

血管内皮生长因子(VEGF)是生长因子的亚家族,具体来说,属于胱氨酸结生长因子中的血小板衍生生长因子(PDGF)家族。它们是重要的信号转导蛋白,参与血管发生(胚胎循环系统从头形成)和血管生成(从已存在的脉管系统生长形成血管)。VEGF可刺激血管发生和血管生成。当血液循环不足时,可参与回复组织供氧系统。VEGF最主要的作用是血管生成。在胚胎形成期,VEGF调控内皮细胞的增殖、迁移和存活,从而调控血管密度和大小,但无法决定血管的类型。在支气管哮喘和糖尿病患者的血清中含有高浓度VEGF。当VEGF过表达时会引起疾病。供血不足时,实体肿瘤的大小受限;而表达VEGF的肿瘤则会不断生长并发生转移。VEGF过表达会导致眼睛视网膜和身体其他部分的血管性疾病。贝伐珠单抗和雷珠单抗可抑制VEGF,并控制或减缓疾病进程。

人 Human VEGFA 分子靶点信息

  • 分子名:VEGFA, vascular endothelial growth factor A
  • 别称:
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • vascular endothelial growth factor A121
    • vascular endothelial growth factor A165
    • vascular permeability factor
    • VEGF
    • VEGF-A
    • VPF
  • 基因序列:NCBI_Gene: 7422
  • 蛋白序列:UniProtKB: P15692

人 Human VEGFA靶点分子功能(预测)

Enables several functions, including fibronectin binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell migration; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including branching involved in blood vessel morphogenesis; positive regulation of endothelial cell proliferation; and regulation of nitric oxide mediated signal transduction. Located in several cellular components, including adherens junction; cell surface; and secretory granule. Implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); inflammatory bowel disease (multiple); and respiratory system cancer (multiple).

操作步骤

检验步骤概要

  1. 1)标准品孔加入100 μL 2倍倍比稀释的标准品。空白孔加入100 μL 标准品稀释液或培养基。
  2. 2)血清/血浆:样本孔加入80 μL 标准品稀释液和20 μL样本。细胞培养上清:样本孔加入100 μL细胞培养上清。
  3. 3)室温(25℃±3℃)孵育1.5小时。撕膜,洗涤3次。
  4. 每孔加入 100 μL 显色底物,避光,室温(25℃±3℃)孵育 10±5 分钟。
  5. 每孔加入 100 μL 终止液。
  6. 30 分钟内,在 450nm 波长检测 OD 值,参考波长 570nm 或 630nm。

检测步骤简略图示

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