EK383HS

Rat VEGF High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：VEGFA, VEGF, VPF
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.34 pg/mL
检测范围：3.13 pg/mL - 200 pg/mL
回收率：91% - 111%

在售SKU:70-EK383HS-48, 70-EK383HS-96

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库存号

描述

文章目录[隐藏]

分子信息
VEGFA 分子靶点信息概述
VEGFA 分子靶点综述
- 大鼠 Rat Vegfa 分子靶点信息
- 大鼠 Rat Vegfa 靶点分子功能（预测）

商品名	Rat VEGF High Sensitivity ELISA Kit (大鼠血管内皮生长因子高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.2% - 4.8%；板间变异系数：4.6% - 5.0%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.34 pg/mL
检测范围	3.13 pg/mL - 200 pg/mL
回收率	91% - 111%
平均回收率	1.02
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗VEGFA单克隆抗体的96孔聚苯乙烯酶标板大鼠VEGFA冻干标准品 VEGFA检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠VEGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的VEGF与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中VEGF的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat VEGF High Sensitivity ELISA Kit (大鼠血管内皮生长因子高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述大鼠 VEGFA, VEGF, VPF 靶点信息

VEGFA 分子靶点信息概述

分子名：VEGFA, vascular endothelial growth factor A
基因家族：VEGF family
别名：VEGF-A; VPF
曾用名：VEGF
全称：vascular endothelial growth factor

VEGFA 分子靶点综述

血管内皮生长因子(VEGF)是生长因子的亚家族，具体来说，属于胱氨酸结生长因子中的血小板衍生生长因子(PDGF)家族。它们是重要的信号转导蛋白，参与血管发生(胚胎循环系统从头形成)和血管生成(从已存在的脉管系统生长形成血管)。VEGF可刺激血管发生和血管生成。当血液循环不足时，可参与回复组织供氧系统。VEGF最主要的作用是血管生成。在胚胎形成期，VEGF调控内皮细胞的增殖、迁移和存活，从而调控血管密度和大小，但无法决定血管的类型。在支气管哮喘和糖尿病患者的血清中含有高浓度VEGF。当VEGF过表达时会引起疾病。供血不足时，实体肿瘤的大小受限；而表达VEGF的肿瘤则会不断生长并发生转移。VEGF过表达会导致眼睛视网膜和身体其他部分的血管性疾病。贝伐珠单抗和雷珠单抗可抑制VEGF，并控制或减缓疾病进程。

大鼠 Rat Vegfa 分子靶点信息

分子名：Vegfa, vascular endothelial growth factor A
别称：
- vascular endothelial growth factor
- vascular permeability factor
- Vegf
- VEGF-A
- VEGF111
- VEGF164
- VPF
基因序列：NCBI_Gene: 83785
蛋白序列：

大鼠 Rat Vegfa 靶点分子功能（预测）

Enables several functions, including identical protein binding activity; receptor ligand activity; and vascular endothelial growth factor receptor 2 binding activity. Involved in several processes, including negative regulation of cell death; response to steroid hormone; and response to vitamin. Located in basement membrane; extracellular space; and vesicle. Used to study several diseases, including brain edema; limb ischemia; middle cerebral artery infarction; ovarian hyperstimulation syndrome; and proliferative diabetic retinopathy. Biomarker of several diseases, including adhesions of uterus; attention deficit hyperactivity disorder; brain edema; liver disease (multiple); and retinal vascular disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).