EK1413

Human PRG2 ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

  • 分子靶点:PRG2
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清/血浆:100 μL 预稀释样本;细胞培养上清:100 μL
  • 灵敏度:22.59 pg/mL
  • 检测范围:39.06 pg/mL - 2500 pg/mL
  • 回收率:80% - 119%

在售SKU:70-EK1413-48, 70-EK1413-96,EK1413

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描述

商品名

Human PRG2 ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

双抗夹心法

精密度

板内变异系数:4.8% - 6.9%;板间变异系数:3.2% - 3.6%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清/血浆:100 μL 预稀释样本;细胞培养上清:100 μL

灵敏度

22.59 pg/mL

检测范围

39.06 pg/mL - 2500 pg/mL

回收率

80% - 119%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 酶标板
  • 标准品
  • 检测抗体
  • 标准品稀释液
  • 链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物 (TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长 (参考波长 570 - 630 nm) 测定吸光度值。

Human PRG2 ELISA Kit 检测试剂盒 - 标准曲线
标准曲线

分子信息

概述 人PRG2, proteoglycan 2, pro eosinophil major basic protein靶点信息

PRG2 分子靶点信息概述

  • 分子名:PRG2, proteoglycan 2, pro eosinophil major basic protein
  • 基因家族:C-type lectin domain containing
  • 曾用名:proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
  • 别名:MBP; BMPG; proMBP
  • 全称:eosinophil major basic protein

PRG2 分子靶点综述

蛋白多糖2 (Proteoglycan 2, PRG2),也叫骨髓蛋白多糖(Bone marrow proteoglycan)、嗜酸性粒细胞颗粒关键碱性蛋白(proeosinophil major basic protein)等。该基因编码的PRG2蛋白质是嗜酸性粒细胞颗粒结晶核心的主要成分。这种蛋白质的高水平前体也存在于胎盘和妊娠血清中,它与几种其他蛋白质形成复合物,包括妊娠相关血浆蛋白A (pregnancy-associated plasma protein A, PAPPA)、血管紧张素原 (angiotensinogen, AGT) 和C3dg。该蛋白可能作为细胞毒素和蠕虫毒素参与抗寄生虫防御机制,以及免疫超敏反应。该编码的蛋白质含有一种肽,对革兰氏阳性细菌、革兰氏阴性细菌和真菌表现出有效的抗菌活性。它直接涉及过敏性疾病中的上皮细胞损伤、剥脱和支气管痉挛。已发现该基因编码不同亚型的选择性剪接转录物变体。

PRG2, proteoglycan 2, pro eosinophil major basic protein 分子靶点种属信息

  • 分子名:PRG2, proteoglycan 2, pro eosinophil major basic protein
  • 别称:
    • BMPG
    • bone marrow proteoglycan
    • bone-marrow proteoglycan
    • eosinophil granule major basic protein
    • eosinophil major basic protein
    • MBP
    • MBP1
    • MGC14537
    • natural killer cell activator
    • proMBP
    • proteoglycan 2 preproprotein
    • proteoglycan 2, bone marrow
    • proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
  • 基因序列:NCBI_Gene: 5553
  • 蛋白序列:UniProtKB: P13727

人 Human PRG2靶点分子功能(预测)

An extracellular matrix structural constituent conferring compression resistance. Predicted to be involved in defense response to bacterium and immune response. Predicted to act upstream of or within defense response to nematode and regulation of cytokine production. Located in collagen-containing extracellular matrix and extracellular exosome. Biomarker of Chagas disease; asthma; and peritoneum cancer.

操作步骤


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