EK1411

Human SOD1 ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:SOD1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:5 μL;细胞培养上清:100 μL
  • 灵敏度:160.37pg/mL
  • 检测范围:0.31 ng/mL - 20 ng/mL
  • 回收率:80% - 120%

在售SKU:70-EK1411-48, 70-EK1411-96

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描述

商品名

Human SOD1 ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

双抗夹心法

精密度

板内变异系数:5.0% - 9.1%;板间变异系数:5.0% - 6.5%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:5 μL;细胞培养上清:100 μL

灵敏度

160.37 pg/mL

检测范围

0.31 ng/mL - 20 ng/mL

回收率

80% - 120%

平均回收率

0.96

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 酶标板
  • 标准品
  • 检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Human SOD1 ELISA Kit 检测试剂盒 - 标准曲线
标准曲线

分子信息

概述人 SOD1, superoxide dismutase 1 靶点信息

SOD1 分子靶点信息概述

  • 分子名:SOD1, superoxide dismutase 1
  • 基因家族:Superoxide dismutases
  • 别名:ALS; ALS1
  • 曾用名:IPOA
  • 全称:amyotrophic lateral sclerosis 1 (adult); superoxide dismutase 1, soluble

SOD1 分子靶点综述

超氧化物歧化酶(Superoxide Dismutase, SOD1)是一种新型酶制剂。它在生物界的分布极广,几乎从动物到植物,甚至从人到单细胞生物,都有它的存在。SOD1属于Cu-Zn超氧化物歧化酶家族。它结合铜和锌离子,是两种同工酶之一,负责清除体内的自由超氧自由基。其编码的同工酶是一种可溶的细胞质蛋白,以同型二聚体的形式将自然产生但有害的超氧化物自由基转化为分子氧和过氧化氢。这个基因的突变被认为是家族性肌萎缩侧索硬化的原因。

人 Human SOD1 分子靶点信息

  • 分子名:SOD1, superoxide dismutase 1
  • 别称:
    • ALS
    • ALS1
    • Cu/Zn superoxide dismutase
    • epididymis secretory protein Li 44
    • HEL-S-44
    • homodimer
    • hSod1
    • indophenoloxidase A
    • IPOA
    • SOD
    • SOD, soluble
    • STAHP
    • superoxide dismutase
    • superoxide dismutase [Cu-Zn]
    • superoxide dismutase 1, soluble
    • superoxide dismutase, cystolic
  • 基因序列:NCBI_Gene: 6647
  • 蛋白序列:UniProtKB: P00441

人 Human SOD1靶点分子功能(预测)

Enables several functions, including enzyme binding activity; superoxide dismutase activity; and transition metal ion binding activity. Involved in several processes, including hydrogen peroxide biosynthetic process; negative regulation of cholesterol biosynthetic process; and positive regulation of superoxide anion generation. Located in several cellular components, including dendrite cytoplasm; neuronal cell body; and peroxisome. Part of protein-containing complex. Colocalizes with plasma membrane. Implicated in several diseases, including Down syndrome; artery disease (multiple); eye disease (multiple); glucose metabolism disease (multiple); and neurodegenerative disease (multiple). Biomarker of several diseases, including Behcet's disease; artery disease (multiple); cutaneous leishmaniasis; eye disease (multiple); and neurodegenerative disease (multiple).

操作步骤


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