EK1227

Human BID ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:BID
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清等
  • 检测样本体积:5 μL
  • 灵敏度:0.51 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:93% - 109%

在售SKU:70-EK1227-48, 70-EK1227-96, EK1227

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描述

商品名

Human BID ELISA Kit 检测试剂盒(酶联免疫吸附法)ELISA试剂盒

检测方法

双抗夹心法

精密度

板内变异系数:2.3% - 3.0%;板间变异系数:1.7% - 2.2%

样本类型

血清、血浆,细胞培养上清等

检测样本体积

5 μL

灵敏度

0.51 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

93% - 109%

平均回收率

1.02

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被特异性抗人 BID 抗体的96孔聚苯乙烯酶标板
  • Human BID冻干标准品
  • Streptavidin-HRP标记Human BID单克隆检测抗体
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜
  • 标准品稀释液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人 BID 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的 BID 与固相抗体和检测抗体结合。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中 BID 的浓度成正比。加入终止液终止反应,在450 nm波长 (参考波长 570 - 630 nm)测定吸光度值。

Human BID ELISA Kit ELISA试剂盒 - 标准曲线
标准曲线

分子信息

概述人 Human BID 分子靶点信息

BID 分子靶点信息概述

  • 分子名:BID, BH3 interacting domain death agonist
  • 基因家族:Receptor ligands; BCL2 homology region 3 (BH3) only; MicroRNA protein coding host genes

BID 分子靶点综述

BH3 结构域凋亡诱导蛋白(BID)是 Bcl-2 蛋白家族中的促凋亡成员,只含有 BH3 结构域。BID 对由Bcl-2 家族蛋白酶介导的细胞死亡很重要,是蛋白酶介导的死亡信号的哨兵。在凋亡信号应答中,BID 与另一个 Bcl-2 家族蛋白Bax 相互作用,导致 Bax 插入到细胞器膜上,主要是线粒体外膜。肿瘤抑制基因 p53 可上调 BID 的表达,BID 已被证明可参与p53 介导的细胞凋亡。最近的研究表明,BID 不仅仅是一个杀手分子,它还通过检查有丝分裂来参与维持基因组的稳定性。BID 是内在死亡途径的关键调节者,可增强 caspase 依赖性的和非依赖性的神经元凋亡的实行。因此,药物抑制BID可为治疗神经系统疾病提供一种很有前途的治疗策略,因为神经系统中的程序性细胞死亡很明显。

人 Human BID 分子靶点信息

  • 分子名:BID, BH3 interacting domain death agonist
  • 别称:
    • apoptic death agonist
    • BH3 interacting domain death agonist Si6 isoform
    • BH3-interacting domain death agonist
    • BID isoform ES(1b)
    • BID isoform L(2)
    • BID isoform Si6
    • desmocollin type 4
    • FP497
    • Human BID coding sequence
    • MGC15319
    • MGC42355
    • p22 BID
  • 基因序列:NCBI_Gene: 637
  • 蛋白序列:UniProtKB: P55957

人 Human BID靶点分子功能(预测)

Predicted to enable death receptor binding activity. Involved in several processes, including hepatocyte apoptotic process; positive regulation of apoptotic signaling pathway; and positive regulation of cellular component organization. Located in cytosol and mitochondrial outer membrane. Implicated in pancreatic cancer.

检测步骤


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