EK1194

Human OSMR ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:OSMR
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:20 μL;细胞培养上清:100 μL
  • 灵敏度:35.9 pg/mL
  • 检测范围:0.16 ng/mL - 10 ng/mL
  • 回收率:81% - 112%

在售SKU:70-EK1194-48, 70-EK1194-96,EK1194,70-EK1194/2-48, 70-EK1194/2-96,EK1194/2

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描述

商品名

Human OSMR ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

双抗夹心法

精密度

板内变异系数:3.4% - 4.3%;板间变异系数:5.2% - 7.0%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:20 μL;细胞培养上清:100 μL

灵敏度

35.9 pg/mL

检测范围

0.16 ng/mL - 10 ng/mL

回收率

81% - 112%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 酶标板
  • 标准品
  • 检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人 OSMR 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记检测抗体,经过孵育,样本中存在的 OSMR 与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中 OSMR 的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Human OSMR ELISA Kit 检测试剂盒 - 标准曲线
标准曲线

分子信息

概述人 OSMR, oncostatin M receptor 靶点信息

OSMR 分子靶点信息概述

  • 分子名:OSMR, oncostatin M receptor
  • 基因家族:Fibronectin type III domain containing
  • 别名:OSMRB; OSMRbeta
  • 全称:Oncostatin-M-specific receptor subunit beta

OSMR 分子靶点综述

抑瘤素M受体(Onconstatin M receptor, OSMR)是白细胞介素-6受体家族成员之一,该家族执行多种细胞功能,在炎症反应,代谢性疾病及细胞生长分化等生物学过程中起着重要作用。OSMR与gp130结合形成对OSM具有高亲和度的受体。在胃癌、结直肠癌、乳腺癌及胶质瘤等癌症样本中均可检测到OSM及OSMR的过度表达,因此靶向 OSMR 是一种治疗各种癌症以及皮肤疾病的重要治疗手段。

人 Human OSMR 分子靶点信息

  • 分子名:OSMR, oncostatin M receptor
  • 别称:
    • IL-31 receptor subunit beta
    • IL-31R subunit beta
    • IL-31R-beta
    • IL-31RB
    • interleukin-31 receptor subunit beta
    • MGC150626
    • MGC150627
    • MGC75127
    • oncostatin-M specific receptor beta subunit
    • oncostatin-M-specific receptor subunit beta
    • OSMRB
    • OSMRbeta
    • PLCA1
  • 基因序列:NCBI_Gene: 9180
  • 蛋白序列:UniProtKB: Q99650

人 Human OSMR靶点分子功能(预测)

Enables growth factor binding activity. Contributes to oncostatin-M receptor activity. Involved in oncostatin-M-mediated signaling pathway and positive regulation of cell population proliferation. Part of apical plasma membrane and oncostatin-M receptor complex. Implicated in primary localized cutaneous amyloidosis 1.

操作步骤


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