F21279A03

Anti-Mouse CD279(PD-1)，APC (Clone: RMP1-30) 流式抗体 (新品)

¥1,224.00 – ¥2,712.00

说明书

分子靶点：PDCD1, PD1, CD279
种属：小鼠 (Mouse)
标记：APC
亚型：Rat IgG2b, κ
克隆号：RMP1-30

在售SKU, 70-AM27905-20, 70-AM27905-50, 70-AM27905-100, AM27905, 70-F21279A03-25, 70-F21279A03-100

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Size
库存号

描述

文章目录[隐藏]

分子信息
PDCD1 分子靶点信息概述
PDCD1 分子靶点综述
- 小鼠 Mouse Pdcd1 分子靶点信息
- 小鼠 Mouse Pdcd1 靶点分子功能（预测）

商品名	Anti-Mouse CD279(PD-1)，APC (Clone: RMP1-30) 流式抗体
靶点	PDCD1, PD1, CD279
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	Rat IgG2b, κ
克隆号	RMP1-30
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 图. ICR 小鼠脾细胞染色 Anti-Mouse CD279, APC (AM27905) 和 Anti-MouseCD4, FITC (AM00401)的结果。已排除粘连细胞。

分子信息

概述小鼠 PDCD1, PD1, CD279 靶点信息

PDCD1 分子靶点信息概述

分子名：PDCD1, programmed cell death 1
基因家族：CD molecules; V-set domain containing
别名：CD279; PD1; hSLE1; PD-1
曾用名：SLEB2
全称：systemic lupus erythematosus susceptibility 2

PDCD1 分子靶点综述

CD279，又名程序性细胞死亡蛋白1(PD-1)，由PDCD1基因编码。PD1为免疫球蛋白超家族成员，与CD28、CTLA-4和ICOS分子同属于CD28超家族，主要以单体形式表达于活化的CD4+T和CD8+T细胞及B细胞，其配体B7-H1和B7-DC。研究证实，增强PD-1的信号可以下调已活化的T、B细胞在外周组织中的应答，在维持外周T、B细胞耐受中发挥重要的作用。

小鼠 Mouse Pdcd1 分子靶点信息

分子名：Pdcd1, programmed cell death 1
别称：
- PD-1
- Pdc1
- programmed cell death
基因序列：NCBI_Gene: 18566
蛋白序列：
- UniprotKB: Q02242
- UniprotKB: Q544F3

小鼠 Mouse Pdcd1 靶点分子功能（预测）

Involved in negative regulation of immune response. Acts upstream of or within negative regulation of apoptotic process; negative regulation of tolerance induction; and positive regulation of apoptotic process. Located in external side of plasma membrane. Is expressed in retina. Used to study dilated cardiomyopathy and systemic lupus erythematosus. Human ortholog(s) of this gene implicated in autoimmune disease (multiple); hepatitis B; hepatitis C; hepatocellular carcinoma; and lupus nephritis. Orthologous to human PDCD1 (programmed cell death 1).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Mouse CD279, APC 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300× g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Mouse CD279, APC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。