EK302HS

Rat IL-2 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：IL2, IL-2
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.71 pg/mL
检测范围：12.5 pg/mL - 800 pg/mL
回收率：95% - 119%

在售SKU:70-EK302HS-48, 70-EK302HS-96

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Size
库存号

描述

文章目录[隐藏]

分子信息
IL2 分子靶点信息概述
IL2 分子靶点综述
- 大鼠 Rat Il2 分子靶点信息
- 大鼠 Rat Il2 靶点分子功能（预测）

商品名	Rat IL-2 High Sensitivity ELISA Kit (大鼠白细胞介素2高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.0%-4.4%；板间变异系数：3.9% - 4.8%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.71 pg/mL
检测范围	12.5 pg/mL - 800 pg/mL
回收率	95% - 119%
平均回收率	1.03
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-2单克隆抗体的96孔聚苯乙烯酶标板大鼠IL-2冻干标准品 IL-2检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-2与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-2 High Sensitivity ELISA Kit (大鼠白细胞介素2高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述大鼠 IL2, IL-2 靶点信息

IL2 分子靶点信息概述

分子名：IL2, interleukin 2
基因家族：Interleukins
别名：IL-2; TCGF
全称：T cell growth factor

IL2 分子靶点综述

白细胞介素2(IL-2)是15kDa的糖蛋白，在人类中，由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子，调控对具有免疫功能的白血球(白细胞，通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答，区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能，其受体由3条链组成，分别为α链(CD25)、β链(CD122)和γ链(CD132)。监测血清中的IL-2含量为多种病理状态提供更详细的视角，包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。

大鼠 Rat Il2 分子靶点信息

分子名：Il2, interleukin 2
别称：
- IL-2
- interleukin-2
- T-cell growth factor
- TCGF
基因序列：NCBI_Gene: 116562
蛋白序列：UniProtKB: P17108

大鼠 Rat Il2 靶点分子功能（预测）

Enables carbohydrate binding activity; glycosphingolipid binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of T cell activation; protein kinase C-activating G protein-coupled receptor signaling pathway; and regulation of protein phosphorylation. Located in extracellular space. Used to study cystitis. Biomarker of allergic rhinitis; colorectal carcinoma; and tongue squamous cell carcinoma. Human ortholog(s) of this gene implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).