EK2107

Mouse LIF ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：LIF, CDF, DIA
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：50 μL
灵敏度：0.77 pg/mL
检测范围：7.81 pg/mL - 500 pg/mL
回收率：91% - 120%

在售SKU:70-EK2107-48, 70-EK2107-96

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库存号

描述

文章目录[隐藏]

分子信息
LIF 分子靶点信息概述
LIF 分子靶点综述
- 小鼠 Mouse Lif 分子靶点信息
- 小鼠 Mouse Lif 靶点分子功能（预测）

商品名	Mouse LIF ELISA Kit (小鼠白血病抑制因子 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.8% - 4.8%；板间变异系数：3.9% - 5.9%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	50 μL
灵敏度	0.77 pg/mL
检测范围	7.81 pg/mL - 500 pg/mL
回收率	91% - 120%
平均回收率	1.08
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗LIF单克隆抗体的96孔聚苯乙烯酶标板人LIF冻干标准品人LIF检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠LIF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的LIF与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中LIF的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse LIF ELISA Kit (小鼠白血病抑制因子 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 LIF, CDF, DIA 靶点信息

LIF 分子靶点信息概述

分子名：LIF, LIF interleukin 6 family cytokine
基因家族：Receptor ligands; Interleukin 6 type cytokine family
别名：CDF; DIA; HILDA
全称：differentiation inhibitory activity; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; human interleukin in DA cells; leukemia inhibitory factor; LIF, interleukin 6 family cytokine

LIF 分子靶点综述

白血病抑制因子(LIF)是一种白细胞介素6家族细胞因子，通过抑制分化影响细胞生长。当LIF水平下降时，细胞就会分化。活化的T细胞、单核细胞、星形胶质细胞、成骨细胞、角质形成细胞、肥大细胞和成纤维细胞都可表达LIF。LIF因其能诱导髓系白血病细胞的终端分化，阻止白血病细胞的持续增长而得名。LIF的其它特性包括促进生长、促进不同类型的靶细胞分化、影响骨代谢、恶病质、神经发育、胚胎发育和炎症。LIF参与孕期子宫内膜蜕膜化和胚胎植入子宫内膜的过程。女性体内的LIF和其它细胞因子水平下降时，就容易怀孕，但是不明原因复发性流产的风险会增加。研究表明重组人LIF可能有助于改善女性不明原因不孕不育的着床率。另外，LIF通常用来添加到干细胞培养基中来减少自发分化。

小鼠 Mouse Lif 分子靶点信息

分子名：Lif, leukemia inhibitory factor
别称：
- 无
基因序列：NCBI_Gene: 16878
蛋白序列：

小鼠 Mouse Lif 靶点分子功能（预测）

Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including animal organ development; generation of neurons; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; cranium; early conceptus; and female reproductive system. Orthologous to human LIF (LIF interleukin 6 family cytokine).