EK318

Rat IL-18 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:IL18, IGIF, IL1F4
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:10 μL;细胞培养上清:100 μL
  • 灵敏度:3.50 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:86% - 119%

在售SKU:70-EK318-48, 70-EK318-96,EK318,il-18

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描述

商品名

Rat IL-18 ELISA Kit (大鼠白细胞介素18 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:3.3% - 4.8%;板间变异系数:3.5% - 5.3%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:10 μL;细胞培养上清:100 μL

灵敏度

3.50 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

86% - 119%

平均回收率

1.00

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 酶标板
  • 标准品
  • 检测抗体
  • 链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物 (TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长 (参考波长 570 - 630 nm) 测定吸光度值。

Rat IL-18 ELISA Kit (大鼠白细胞介素18 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 IL18, IGIF, IL1F4 靶点信息

IL18 分子靶点信息概述

  • 分子名:IL18, interleukin 18
  • 基因家族:Interleukins
  • 别名:IGIF; IL1F4; IL-1g; IL-18
  • 全称:interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)

IL18 分子靶点综述

白细胞介素18(IL-18),又名干扰素γ诱导因子,是一种促炎性细胞因子,在人类中由IL18基因编码。IL-18属于IL-1超家族,由巨噬细胞和其它细胞分泌产生。IL-18刺激后,自然杀伤细胞(NK)和某些T细胞就会释放另一种重要的细胞因子—干扰素γ或Ⅱ型干扰素,进而激活巨噬细胞或其它细胞。IL-18可诱导产生严重的炎症反应,同样可增加人类神经元中β-淀粉样蛋白(与阿尔茨海默氏病相关)的产生。

大鼠 Mouse Il18 分子靶点信息

  • 分子名:Il18, interleukin 18
  • 别称:
    • IFN-gamma-inducing factor
    • IL-1 gamma
    • IL-18
    • interferon gamma-inducing factor
    • interferon-gamma-inducing factor
    • interleukin-1 gamma
    • interleukin-18
  • 基因序列:NCBI_Gene: 29197
  • 蛋白序列:

大鼠 Rat Il18 靶点分子功能(预测)

Predicted to enable cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including cellular response to hormone stimulus; positive regulation of cell population proliferation; and positive regulation of cytokine production. Located in apical plasma membrane; cytoplasm; and extracellular space. Used to study brain edema; fatty liver disease; heart disease; status epilepticus; and vasculitis. Biomarker of several diseases, including cardiac arrest; ischemia (multiple); lung disease (multiple); perinatal necrotizing enterocolitis; and urinary tract infection. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).

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