EK184

Human sE-Selectin ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：SELE, ELAM, CD62E
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：10 μL
灵敏度：1.94 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：84% - 120%

在售SKU:70-EK184-48, 70-EK184-96

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库存号

描述

文章目录[隐藏]

分子信息
SELE 分子靶点信息概述
SELE 分子靶点综述
- 人 Human SELE 分子靶点信息
- 人 Human SELE靶点分子功能（预测）

商品名	Human sE-Selectin ELISA Kit (人可溶性E选择素 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.9% - 5.4%；板间变异系数：3.7% - 7.7%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	10 μL
灵敏度	1.94 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	84% - 120%
平均回收率	1.02
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗sE-Selectin单克隆抗体的96孔聚苯乙烯酶标板人sE-Selectin冻干标准品 sE-Selectin检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sE-Selectin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的sE-Selectin与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中sE-Selectin的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human sE-Selectin ELISA Kit (人可溶性E选择素 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 SELE, ELAM, CD62E 靶点信息

SELE 分子靶点信息概述

分子名：SELE, selectin E
基因家族：CD molecules; Selectins; Sushi domain containing; C-type lectin domain containing
别名：ESEL; CD62E
曾用名：ELAM1; ELAM
全称：endothelial adhesion molecule 1

SELE 分子靶点综述

E-选择素，又名CD62E、内皮-白细胞粘附分子1(ELAM-1)或者白细胞-内皮细胞粘附分子2(LECAM2)，是一个115kDa的糖蛋白，只有被炎性细胞因子(IL-1β或TNF-α)或内皮细胞中的内毒素激活后才表达。细胞表面的E-选择素介导白细胞附着到内皮，从而在局部炎症反应中起重要作用。E-选择素的胞外区包括一个钙依赖性的C2型凝集素结构域、一个表皮生长因子(EGF)结构域和6个重复的补体调节蛋白样序列。可溶性E-选择素(sE-Selectin)存在于健康机体的血液中，可能产生于表面表达分子的蛋白裂解。监测血清中sE-Selectin水平可为一些病理情况如癌症、感染性疾病、糖尿病和脓毒病提供更详细的见解。

人 Human SELE 分子靶点信息

分子名：SELE, selectin E
别称：
- CD62 antigen-like family member E
- CD62E
- E-selectin
- ELAM
- ELAM-1
- ELAM1
- endothelial adhesion molecule 1
- endothelial leukocyte adhesion molecule 1
- ESEL
- LECAM2
- leukocyte endothelial cell adhesion molecule 2
- leukocyte-endothelial cell adhesion molecule 2
基因序列：NCBI_Gene: 6401
蛋白序列：UniProtKB: P16581

人 Human SELE靶点分子功能（预测）

Enables several functions, including oligosaccharide binding activity; phospholipase binding activity; and sialic acid binding activity. Involved in several processes, including activation of phospholipase C activity; heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; and leukocyte tethering or rolling. Located in several cellular components, including caveola; clathrin-coated pit; and perinuclear region of cytoplasm. Is integral component of plasma membrane. Implicated in IgA glomerulonephritis; brain ischemia; cerebrovascular disease; and coronary artery disease. Biomarker of COVID-19; rheumatic myocarditis; and type 1 diabetes mellitus.