F110I204

Anti-Human IL-2, PerCP-Cy5.5 (Clone: MQ1-17H12) 检测试剂

¥2,010.00 – ¥4,360.00

说明书

分子靶点：IL2, IL-2
种属：人 (Human)
标记：PerCP-Cy5.5
亚型：Rat IgG2a, κ
克隆号：MQ1-17H12

在售SKU,70-F110I204-25, 70-F110I204-100

¥<\/span>4,360.00<\/bdi><\/span><\/span>","sku":"70-F110I204-100","variation_description":"","variation_id":20129,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"25T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":2010,"display_regular_price":2010,"image":{"title":"1656571413-fc-antibody.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":16568,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>2,010.00<\/bdi><\/span><\/span>","sku":"70-F110I204-25","variation_description":"","variation_id":20130,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
IL2 分子靶点信息概述
IL2 分子靶点综述
- 人 Human IL2 分子靶点信息
- 人 Human IL2靶点分子功能（预测）

商品名	Anti-Human IL-2, PerCP-Cy5.5 (Clone: MQ1-17H12) 检测试剂
靶点	IL2, IL-2
标记	PerCP-Cy5.5
仪器	适用488nm激光器的流式细胞仪
亚型	Rat IgG2a, κ
克隆号	MQ1-17H12
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 正常人的外周血样本用细胞刺激阻断剂（目录号：CS1001和CS1002）预处理，固定破膜剂（目录号：GAS003/2）破膜后进行染色，左图为Rat IgG2a, κ Isotype control, PerCP-Cy5.5和Anti-Human CD3,APC共染的结果，右图为Anti-Human IL-2, PerCP-Cy5.5和Anti-Human CD3, APC共染的结果。以淋巴细胞设门进行分析。

分子信息

概述人 IL2, IL-2 靶点信息

IL2 分子靶点信息概述

分子名：IL2, interleukin 2
基因家族：Interleukins
别名：IL-2; TCGF
全称：T cell growth factor

IL2 分子靶点综述

白细胞介素2(IL-2)是15kDa的糖蛋白，在人类中，由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子，调控对具有免疫功能的白血球(白细胞，通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答，区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能，其受体由3条链组成，分别为α链(CD25)、β链(CD122)和γ链(CD132)。监测血清中的IL-2含量为多种病理状态提供更详细的视角，包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。

人 Human IL2 分子靶点信息

分子名：IL2, interleukin 2
别称：
- aldesleukin
- IL-2
- interleukin-2
- involved in regulation of T-cell clonal expansion
- lymphokine
- T cell growth factor
- TCGF
基因序列：NCBI_Gene: 3558
蛋白序列：UniProtKB: P60568

人 Human IL2靶点分子功能（预测）

Enables cytokine activity and interleukin-2 receptor binding activity. Involved in leukocyte activation involved in immune response; positive regulation of interleukin-17 production; and positive regulation of tyrosine phosphorylation of STAT protein. Acts upstream of or within negative regulation of B cell apoptotic process; positive regulation of B cell proliferation; and positive regulation of activated T cell proliferation. Predicted to be located in extracellular space. Implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Biomarker of several diseases, including Zellweger syndrome; aggressive periodontitis; anogenital venereal wart; autoimmune disease (multiple); and uveitis (multiple).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

请注意该指标为胞内因子，染色前需进行固定破膜操作。

全血样本：取100μL全血样本，经裂红、固定破膜处理后，加入5μL Anti-Human IL-2, PerCP-Cy5.5和其他胞内抗体，震荡混匀，于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。每管加入1-2mL 流式染色缓冲液（目录号：S1001），300×g 离心 5 - 10分钟，弃上清，加入500μL 流式染色缓冲液重悬，上机检测。

其他样本：将 1×10⁵ - 10⁸ 个单细胞经裂红（若需）、固定破膜处理后，取100uL，加入5μL AntiHuman IL-2, PerCP-Cy5.5和其他胞内抗体，使反应终体积为 100μL 。震荡混匀，于室温避光孵育15分钟或 2 - 8℃避光孵育30分钟。每管加入 1 - 2mL 流式染色缓冲液，300 × g离心 5 - 10分钟，弃上清，加入 500μL 流式染色缓冲液重悬，上机检测。

实验中，可对抗体进行优化滴定以获得最佳效果。