EK194

Human C-Reactive Protein ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：CRP, C-reactive protein, PTX1
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL
灵敏度：0.44 pg/mL
检测范围：62.5 pg/mL - 4000 pg/mL
回收率：93% - 107%

在售SKU:70-EK194-48, 70-EK194-96

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库存号

描述

文章目录[隐藏]

分子信息
CRP 分子靶点信息概述
CRP 分子靶点综述
- 人 Human CRP 分子靶点信息
- 人 Human CRP靶点分子功能（预测）

商品名	Human C-Reactive Protein/CRP ELISA Kit (人C-反应蛋白 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.9% - 5.5%；板间变异系数：3.0% - 5.8%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL
灵敏度	0.44 pg/mL
检测范围	62.5 pg/mL - 4000 pg/mL
回收率	93% - 107%
平均回收率	1
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗 CRP单克隆抗体的96孔聚苯乙烯酶标板人 CRP冻干标准品 CRP检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CRP抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的CRP与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CRP的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human C-Reactive Protein/CRP ELISA Kit (人C-反应蛋白 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 CRP, C-reactive protein, PTX1 靶点信息

CRP 分子靶点信息概述

分子名：CRP, C-reactive protein
基因家族：Short pentraxins
别名：PTX1
全称：pentraxin 1; C-reactive protein, pentraxin-related

CRP 分子靶点综述

C-反应蛋白(CRP)是在血浆中发现的一种环状、五聚体蛋白。肝脏对巨噬细胞和脂肪细胞释放的因子产生应答后可合成CRP。CRP是一种肝源性的急性蛋白，在巨噬细胞和T细胞分泌白介素6后增加，其生理作用是与死细胞或濒死细胞(和某些类型的细菌)表面的溶血磷脂胆碱结合，以通过C1Q复合物来激活补体系统。它与C-肽(胰岛素)或蛋白C(血凝)不相关。CRP通常作为炎症标志物，当机体受到炎症感染时，CRP水平会上升。最近的研究表明，患者具有较高基础水平的CRP，通常伴随着一个较高糖尿病、高血压和心血管疾病的发生率。

人 Human CRP 分子靶点信息

分子名：CRP, C-reactive protein
别称：
- C-reactive protein, pentraxin-related
- c-reactive protein, petaxin related
- MGC149895
- MGC88244
- pentraxin 1
- PTX1
基因序列：NCBI_Gene: 1401
蛋白序列：UniProtKB: P02741

人 Human CRP靶点分子功能（预测）

Enables several functions, including complement component C1q complex binding activity; low-density lipoprotein particle binding activity; and low-density lipoprotein particle receptor binding activity. Involved in several processes, including negative regulation of macrophage derived foam cell differentiation; positive regulation of superoxide anion generation; and regulation of gene expression. Acts upstream of or within vasoconstriction. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); autoimmune disease (multiple); eye disease (multiple); and lung disease (multiple).