EK1334

Human Clusterin ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:CLU, Clusterin
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL (稀释液)
  • 灵敏度:8.38 pg/mL
  • 检测范围:0.25 ng/mL - 16 ng/mL
  • 回收率:76% - 117%

在售SKU:70-EK1334-48, 70-EK1334-96

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描述

商品名

Human Clusterin ELISA Kit 人丛生蛋白(Clusterin)ELISA试剂盒

检测方法

双抗夹心法

精密度

板内变异系数:3.8 % - 6.2%;板间变异系数:6.9 % - 9.0%

样本类型

血清、血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL (稀释液)

灵敏度

8.38 pg/mL

检测范围

0.25 ng/mL - 16 ng/mL

回收率

76% - 117%

平均回收率

0.98

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被小鼠抗人Clusterin捕获抗体的96孔聚苯乙烯酶标板
  • Human Clusterin冻干标准品
  • Human Clusterin检测抗体
  • Streptavidin-HRP
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜
  • 标准品稀释液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Clusterin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Clusterin与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Clusterin的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Clusterin ELISA Kit 人丛生蛋白(Clusterin)ELISA试剂盒 - 标准曲线
标准曲线

CLU 分子靶点信息概述

  • 分子名:CLU, clusterin
  • 基因家族:Complement system regulators and receptors; MicroRNA protein coding host genes
  • 别名:SGP-2; SP-40; TRPM-2; KUB1; CLU1; CLU2
  • 曾用名:CLI; APOJ
  • 全称:clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J); complement lysis inhibitor; sulfated glycoprotein 2; testosterone-repressed prostate message 2; apolipoprotein J

CLU 分子靶点综述

丛生蛋白(Clusterin)是 1983 年首次由绵羊睾丸膜体液中分离得到的,是一种普遍存在的、高度保守的异源二聚体硫酸化糖蛋白,该蛋白广泛表达于机体多种组织中,发挥促细胞聚集、调节生殖、组织修复、转运脂类、补体抑制/调节免疫等多种功能。由于其具有广泛、未明机制的各种功能,因而具有多种命名,例如载脂蛋白 J、硫酸糖蛋白 2 (SGP-2)、TRPM-2 和 SP-40 等。在 1992 年英国剑桥召开的国际会议上将其统一命名为 Clusterin,简称 CLU。研究表明 Clusterin 不仅与多种生物过程相关,而且还与多种病理状态(如神经退行性疾病、衰老和癌症)有关。人 Clusterin 包含两个螺旋结构域、三个核定位信号域和一个肝素结合域。细胞内前体的裂解去除信号肽并产生相当大小的α链和β链,分泌为大约80kDa 的 N-糖基化和二硫键连接的异二聚体。

CLU, clusterin 分子靶点种属信息

人 Human

人 Human CLU 分子靶点信息

  • 分子名:CLU, clusterin
  • 别称:
    • AAG4
    • aging-associated protein 4
    • APO-J
    • APOJ
    • apolipoprotein J
    • CLI
    • CLU1
    • CLU2
    • complement cytolysis inhibitor
    • complement lysis inhibitor
    • complement-associated protein SP-40,40
    • kubinding protein 1
    • KUB1
    • MGC24903
    • NA1/NA2
    • SGP-2
    • SGP2
    • SP-40
    • sulfated glycoprotein 2
    • testosterone-repressed prostate message 2
    • TRPM-2
    • TRPM2
  • 基因序列:NCBI_Gene: 1191
  • 蛋白序列:UniProtKB: P10909

人 Human CLU靶点分子功能(预测)

Enables several functions, including amyloid-beta binding activity; low-density lipoprotein particle receptor binding activity; and misfolded protein binding activity. Involved in several processes, including positive regulation of cellular component organization; regulation of intrinsic apoptotic signaling pathway; and regulation of protein metabolic process. Located in several cellular components, including apical dendrite; neurofibrillary tangle; and perinuclear endoplasmic reticulum lumen. Part of spherical high-density lipoprotein particle. Colocalizes with cell periphery; cytoskeleton; and membrane. Implicated in artery disease (multiple); breast cancer; diabetic retinopathy; exfoliation syndrome; and muscular disease (multiple). Biomarker of several diseases, including Kawasaki disease; Stevens-Johnson syndrome; autoimmune disease of musculoskeletal system (multiple); eye disease (multiple); and invasive ductal carcinoma.

检测步骤


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