EK382EGB

Easy Go!™ Rat TNF-α One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥2,190.00 – ¥3,650.00

分子靶点：TNF, TNFA, TNFSF2
种属：大鼠 (Rat)
样本类型：细胞培养上清
检测样本体积：100 μL
灵敏度：0.47 pg/mL
检测范围：7.81 - 500 pg/mL
回收率：85%-114%

在售SKU: 70-EK382EGB-48, 70-EK382EGB-96, EK382EGB, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒

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Size
库存号

描述

文章目录[隐藏]

分子信息
TNF 分子靶点信息概述
TNF 分子靶点综述
- 大鼠 Rat Tnf 分子靶点信息
- 大鼠 Rat Tnf 靶点分子功能（预测）

商品名	Easy Go!™ Rat TNF-α One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法	一步法
精密度	板内变异系数：5.3% - 8.2%；板间变异系数：4.5 % - 7.3%
样本类型	细胞培养上清
检测样本体积	100 μL
灵敏度	0.47 pg/mL
检测范围	7.81 - 500 pg/mL
回收率	85% - 114%
平均回收率	101%
板式	96孔板，可拆
保存	试剂盒未拆开，2-8℃保存。已拆开，标准品-20℃保存，其它2-8℃保存。
运输条件	4℃蓝冰运输
组分	含微球的预包被酶标板标准品显色底物终止液 20×洗液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上，酶标板孔中预先加入生物素标记的微球和辣根过氧化物酶微球。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450 nm波长 (参考波长570 - 630 nm) 测定吸光度值。

Easy Go!™ Rat TNF-α One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）- 标准曲线 — 标准曲线

分子信息

概述大鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

分子名：TNF, tumor necrosis factor
基因家族：Tumor necrosis factor superfamily
别名：TNFSF2; DIF; TNF-alpha
曾用名：TNFA
全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

大鼠 Rat Tnf 分子靶点信息

分子名：Tnf, tumor necrosis factor
别称：
- cachectin
- LOC103694380
- MGC124630
- RATTNF
- TNF-a
- TNF-alpha
- Tnfa
- tumor necrosis factor (TNF superfamily, member 2)
- tumor necrosis factor alpha
- tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor superfamily member 2
- tumor necrosis factor superfamily, member 2
- tumor necrosis factor-alpha
- tumor necrosis factor-like
基因序列：NCBI_Gene: 24835
蛋白序列：UniProtKB: P16599

大鼠 Rat Tnf 靶点分子功能（预测）

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).