EK182EGB

EasyGo!™ Human TNF-α One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥2,190.00 – ¥3,650.00

分子靶点：TNF, TNFA, TNFSF2
种属：人 (Human)
样本类型：细胞培养上清
检测样本体积：100 μL
灵敏度：6.58 pg/mL
检测范围：15.63 - 1000 pg/mL
回收率：75% - 125%

在售SKU: 70-EK182EGB-48, 70-EK182EGB-96, EK182EGB, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒

¥<\/span>2,190.00<\/bdi><\/span><\/span>","sku":"70-EK182EGB-48","variation_description":"","variation_id":34627,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"96T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":3650,"display_regular_price":3650,"image":{"title":"EasyGo-B","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-B.png","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-B-800x600.png","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-B-800x600.png 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-B-768x576.png 768w, https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-B.png 1024w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-B.png","full_src_w":1024,"full_src_h":768,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-B-100x100.png","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-B.png","thumb_src_w":1024,"thumb_src_h":768,"src_w":800,"src_h":600},"image_id":23645,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>3,650.00<\/bdi><\/span><\/span>","sku":"70-EK182EGB-96","variation_description":"","variation_id":34628,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

Size
库存号

描述

文章目录[隐藏]

分子信息
TNF 分子靶点信息概述
TNF 分子靶点综述
- 人 Human TNF 分子靶点信息
- 人 Human TNF靶点分子功能（预测）

商品名	EasyGo!™ HumanTNF-α One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法	一步法
精密度	板内变异系数：6.7%-9.1%；板间变异系数：5.3%-7.3%
样本类型	细胞培养上清
检测样本体积	100 μL
灵敏度	6.58 pg/mL
检测范围	15.63 - 1000 pg/mL
回收率	75% - 125%
平均回收率	95%
板式	96孔板，可拆
保存	试剂盒未拆开，2-8℃保存。已拆开，标准品-20℃保存，其它2-8℃保存。
运输条件	4℃蓝冰运输
组分	含微球的预包被酶标板标准品显色底物终止液 20×洗液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上，酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450 nm波长 (参考波长570 - 630 nm) 测定吸光度值。

EasyGo!™ HumanTNF-α One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）- 标准曲线 — 标准曲线

分子信息

概述人 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

分子名：TNF, tumor necrosis factor
基因家族：Tumor necrosis factor superfamily
别名：TNFSF2; DIF; TNF-alpha
曾用名：TNFA
全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

人 Human TNF 分子靶点信息

分子名：TNF, tumor necrosis factor
别称：
- APC1 protein
- cachectin
- DIF
- TNF superfamily, member 2
- TNF-a
- TNF-alpha
- TNF, macrophage-derived
- TNF, monocyte-derived
- TNFA
- TNFSF2
- TNLG1F
- tumor necrosis factor alpha
- tumor necrosis factor ligand 1F
- tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor-alpha
基因序列：NCBI_Gene: 7124
蛋白序列：UniProtKB: P01375

人 Human TNF靶点分子功能（预测）

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).