EK213

Mouse IL-13 ELISA Kit检测试剂盒(酶联免疫吸附法)

X

¥1,600.00¥2,650.00

  • 分子靶点:IL13, ALRH, BHR1
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:1.17 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:89% - 112%

在售SKU:70-EK213/2-48, 70-EK213/2-96

¥<\/span>1,600.00<\/bdi><\/span><\/span>","sku":"70-EK213\/2-48","variation_description":"","variation_id":10559,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"96T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":2650,"display_regular_price":2650,"image":{"title":"ELISA-1.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":5986,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>2,650.00<\/bdi><\/span><\/span>","sku":"70-EK213\/2-96","variation_description":"","variation_id":10560,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

描述

商品名

Mouse IL-13 ELISA Kit (小鼠白细胞介素13 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.3%-3.0%;板间变异系数:2.1% - 5.3%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

1.17 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

89% - 112%

平均回收率

1.02

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-13单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-13冻干标准品
  • IL-13检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-13抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-13与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-13的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-13 ELISA Kit (小鼠白细胞介素13 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL13, ALRH, BHR1 靶点信息

IL13 分子靶点信息概述

  • 分子名:IL13, interleukin 13
  • 基因家族:Interleukins
  • 别名:P600; IL-13; ALRH; BHR1; MGC116786; MGC116788; MGC116789
  • 全称:allergic rhinitis; Bronchial hyperresponsiveness-1 (bronchial asthma)

IL13 分子靶点综述

白细胞介素13(IL-13)位于染色体5q31上,与IL-4具有30%的同源性和相似的结构。IL-13由许多细胞类型分泌产生,尤其是2型辅助性T细胞(Th2)。与密切相关的IL-4相似,IL-13对免疫细胞也具有多种功能。虽然IL-13主要与气道疾病的诱导相关,但是它还具有消炎特性。IL-13可诱导造血细胞的变化,但是这些效果与IL-4相比可能不那么重要。IL-13专门诱导寄生感染的器官的生理变化。它也可诱导过敏性肺部疾病的许多特征,包括气道高反应性、杯状细胞增殖和黏液高分泌,这些都会导致气道阻塞。此外,IL-13抑制原代培养衍生的巨噬细胞中HIV-1的复制,可作为体内单核细胞和巨噬细胞中抑制HIV感染的候选细胞因子。

小鼠 Mouse Il13 分子靶点信息

小鼠 Mouse Il13 靶点分子功能(预测)

Predicted to enable interleukin-13 receptor binding activity. Involved in positive regulation of cold-induced thermogenesis and positive regulation of macrophage activation. Acts upstream of or within cellular response to cytokine stimulus; inflammatory response; and positive regulation of immune effector process. Located in cytoplasm; external side of plasma membrane; and extracellular space. Is expressed in gut; liver; male reproductive gland or organ; and thymus. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Orthologous to human IL13 (interleukin 13).

操作步骤


下载本产品说明书

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献


  1. 支气管肺泡灌洗液 BALF 组织细胞

    Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation 

评价

目前还没有评价

成为第一个“Mouse IL-13 ELISA Kit检测试剂盒(酶联免疫吸附法)” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注