F21IFNG01

Anti-Mouse IFN-γ, FITC (Clone:XMG1.2) 流式抗体 (新品)

¥408.00 – ¥888.00

说明书

分子靶点：IFNG, IFN-γ
种属：小鼠 (Mouse)
标记：FITC
亚型：Rat IgG1, κ
克隆号：XMG1.2

在售SKU, 70-AM0IF01-20, 70-AM0IF01-50, 70-AM0IF01-100, AM0IF01, 70-F21IFNG01-25, 70-F21IFNG01-100

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Size
库存号

描述

文章目录[隐藏]

分子信息
IFNG 分子靶点信息概述
IFNG 分子靶点综述
- 小鼠 Mouse Ifng 分子靶点信息
- 小鼠 Mouse Ifng 靶点分子功能（预测）

商品名	Anti-Mouse IFN-γ, FITC (Clone:XMG1.2) 流式抗体
靶点	IFNG, IFN-γ
标记	FITC
仪器	适用488nm激光器的流式细胞仪
亚型	Rat IgG1, κ
克隆号	XMG1.2
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 图. ICR 小鼠脾细胞未刺激对照组 ( 左 ) 和经PMA/Ionomycin (Cat No. CS1001) 刺激和 BFA/Monensin (Cat No. CS1002)阻断的实验组(右)染色Anti-Mouse CD4, PerCP-Cy5.5 (Cat No. AM00407)和Anti-Mouse IFN-γ, FITC 的结果。以 CD3+细胞设门分析。已排除黏连细胞。

分子信息

概述小鼠 IFNG, IFN-γ 靶点信息

IFNG 分子靶点信息概述

分子名：IFNG, interferon gamma
基因家族：Interferons

IFNG 分子靶点综述

IFN-γ主要由Th1型细胞产生，属于II型细胞因子中干扰素家族中的一员，是一类分泌型糖蛋白，由143个氨基酸组成，相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生，具有调节机体免疫状态，介导细胞免疫，加强受体介导的吞噬功能，增强巨噬细胞和中性粒细胞的杀伤活性，具有抗病毒感染及抗肿瘤等生物学活性。

小鼠 Mouse Ifng 分子靶点信息

分子名：Ifng, interferon gamma
别称：
- Ifg
- IFN-gamma
基因序列：NCBI_Gene: 15978
蛋白序列：

小鼠 Mouse Ifng 靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and regulation of generation of precursor metabolites and energy. Acts upstream of or within several processes, including CD8-positive, alpha-beta T cell differentiation involved in immune response; defense response to other organism; and positive regulation of macromolecule metabolic process. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including adipose tissue; alimentary system; genitourinary system; hemolymphoid system gland; and nervous system. Used to study several diseases, including aplastic anemia; hepatitis; malaria; medulloblastoma; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including allergic rhinitis; ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

将样本经适当处理后，取 100 ?l 含有 1×10⁵ - 10⁸ 个单细胞悬液，加入 5 ?l Anti-Mouse IFN-γ, FITC。震荡混匀，室温避光孵育 15 分钟。如进行 Th1/Th2 检测，可参考 Mouse Th1/Th2 Staining Kit (Cat No.KTH201)中的实验方案。
实验中，可对抗体进行优化滴定以获得最佳效果。