EK205HS

Mouse IL-5 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：IL5, IL-5, TRF
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.14 pg/mL
检测范围：1.56 pg/mL - 100 pg/mL
回收率：104% - 107%

在售SKU:70-EK205HS-48, 70-EK205HS-96

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库存号

描述

文章目录[隐藏]

分子信息
IL5 分子靶点信息概述
IL5 分子靶点综述
- 小鼠 Mouse Il5 分子靶点信息
- 小鼠 Mouse Il5 靶点分子功能（预测）

商品名	Mouse IL-5 High Sensitivity ELISA Kit (小鼠白细胞介素5 (IL-5) 高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.8% - 5.0%；板间变异系数：3.0% - 5.3%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.14 pg/mL
检测范围	1.56 pg/mL - 100 pg/mL
回收率	104% - 107%
平均回收率	1.05
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-5单克隆抗体的96孔聚苯乙烯酶标板小鼠IL-5冻干标准品 IL-5检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素信号增强剂浓缩液信号增强剂稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-5与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-5的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-5 High Sensitivity ELISA Kit (小鼠白细胞介素5 (IL-5) 高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 IL5, IL-5, TRF 靶点信息

IL5 分子靶点信息概述

分子名：IL5, interleukin 5
基因家族：Interleukins
别名：IL-5; EDF; TRF
全称：interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)

IL5 分子靶点综述

白细胞介素5(IL-5)是造血家族Th2细胞因子成员，由115个氨基酸组成。与该细胞因子家族其它成员(IL-3和GM-CSF)不同，该糖蛋白的活化形式是同源二聚体。IL-5由Th2细胞和肥大细胞分泌。它对B细胞以及其它类型的细胞产生的生物学活性表明，IL-5对Th2细胞的活性具有重要的功能性作用。IL-5通过与其受体结合，刺激B细胞生长，提高免疫球蛋白分泌。它也是活化嗜酸性粒细胞的关键因子。IL-5一直以来与多种变态疾病相关，包括变应性鼻炎和哮喘，在循环组织、气管组织中观察到大幅增加的IL-5，而痰嗜酸性粒细胞则减少。

小鼠 Mouse Il5 分子靶点信息

分子名：Il5, interleukin 5
别称：
- Il-5
基因序列：NCBI_Gene: 16191
蛋白序列：

小鼠 Mouse Il5 靶点分子功能（预测）

Enables cytokine activity. Involved in positive regulation of DNA-binding transcription factor activity; positive regulation of immunoglobulin production; and positive regulation of transcription, DNA-templated. Acts upstream of or within positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in colon; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).