EK1255A

Human IGFBP-1 ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

  • 分子靶点:IGFBP1, AFBP, IBP1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清/血浆:100 μL 预稀释样本;细胞培养上清:100 μL
  • 灵敏度:0.59 pg/mL
  • 检测范围:9.38 pg/mL - 600 pg/mL
  • 回收率:82% - 113%

在售SKU:70-EK1255A-48, 70-EK1255A-96,EK1255A

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描述

商品名

Human IGFBP-1 ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

双抗夹心法

精密度

板内变异系数:2.68% - 4.13%;板间变异系数:1.41% - 3.21%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清/血浆:100 μL 预稀释样本;细胞培养上清:100 μL

灵敏度

0.59 pg/mL

检测范围

9.38 pg/mL - 600 pg/mL

回收率

82% - 113%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 酶标板
  • 标准品
  • 检测抗体
  • 链霉亲和素
  • 质控品(高值)
  • 质控品(低值)
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人 IGFBP-1 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的 IGFBP-1 与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中 IGFBP-1 的浓度成正比。加入终止液终止反应,在450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Human IGFBP-1 ELISA Kit 检测试剂盒 - 标准曲线
标准曲线

分子信息

概述人 IGFBP1, AFBP, IBP1 靶点信息

IGFBP1 分子靶点信息概述

  • 分子名:IGFBP1, insulin like growth factor binding protein 1
  • 基因家族:Insulin like growth factor binding proteins
  • 别名:IGF-BP25; AFBP; hIGFBP-1; PP12
  • 曾用名:IBP1
  • 全称:placental protein 12; amniotic fluid binding protein; alpha-pregnancy-associated endometrial globulin; growth hormone independent-binding protein; binding protein-28; binding protein-26; binding protein-25; IGF-binding protein 1; insulin-like growth factor binding protein 1

IGFBP1 分子靶点综述

胰岛素样生长因子-结合蛋白 1(IGFBP-1)也被称为胎盘蛋白 12(PP12),是一种由 IGFBP1 基因 编码的蛋白。它能结合胰岛素样生长因子(IGFs)I 和 II,并在血浆中循环。这样的结合延长了 IGFs 的半衰期,改变了它们与细胞表面受体的相互作用。IGFBPs 可以调节 IGF 蛋白的生物活性。IGFBP 的转录后修饰(如糖基化、磷酸化和蛋白水解)可改变结合蛋白与 IGF 的亲和力。高浓度 IGFBP-1 可能是分解代谢状态的标志,研究表明含高浓度 IGFBP-1 的女性具有较少的肌肉量。鼻咽癌患者 血清中较高的 IGFBP-1/IGF-1 比与预后差显著相关。据报道,局部 IGFBP1 水平对冠状动脉斑块的 形成有影响。

人 Human IGFBP1 分子靶点信息

  • 分子名:IGFBP1, insulin like growth factor binding protein 1
  • 别称:
    • AFBP
    • alpha-pregnancy-associated endometrial globulin
    • amniotic fluid binding protein
    • binding protein-25
    • binding protein-26
    • binding protein-28
    • growth hormone independent-binding protein
    • hIGFBP-1
    • IBP-1
    • IBP1
    • IGF-binding protein 1
    • IGF-BP25
    • IGFBP-1
    • insulin-like growth factor binding protein 1
    • insulin-like growth factor-binding protein 1
    • placental protein 12
    • PP12
  • 基因序列:NCBI_Gene: 3484
  • 蛋白序列:UniProtKB: P08833

人 Human IGFBP1靶点分子功能(预测)

Enables insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in Golgi apparatus. Implicated in diabetic angiopathy. Biomarker of breast cancer.

操作步骤


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