EK198

Human CTGF ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：CCN2, CTGF
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：50 μL
灵敏度：2.31 pg/mL
检测范围：62.5 pg/mL - 4000 pg/mL
回收率：88% - 115%

在售SKU:70-EK198-48, 70-EK198-96

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Size
库存号

描述

文章目录[隐藏]

分子信息
CCN2 分子靶点信息概述
CCN2 分子靶点综述
- 人 Human CCN2 分子靶点信息
- 人 Human CCN2靶点分子功能（预测）

商品名	Human CTGF ELISA Kit (人结缔组织生长因子 (CTGF) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.9% - 6.7%；板间变异系数：6.3% - 7.5%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	50 μL
灵敏度	2.31 pg/mL
检测范围	62.5 pg/mL - 4000 pg/mL
回收率	88% - 115%
平均回收率	1.03
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗CTGF单克隆抗体的96孔聚苯乙烯酶标板人CTGF冻干标准品 CTGF检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CTGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的CTGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CTGF的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human CTGF ELISA Kit (人结缔组织生长因子 (CTGF) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 CCN2, CTGF 靶点信息

CCN2 分子靶点信息概述

分子名：CCN2, cellular communication network factor 2
基因家族：Cellular communication network factors
别名：IGFBP8
曾用名：CTGF
全称：connective tissue growth factor

CCN2 分子靶点综述

结缔组织生长因子(CTGF)是CNN家族中的细胞基质蛋白。CTGF的胞外部分包括四个保守的、富含半胱氨酸的结构域：胰岛素样生长因子结合蛋白(IGFBP)结构域、血管性血友病C型重复序列(vWC)结构域、血小板反应蛋白1型重复序列(TSR)结构域以及含有半胱氨酸基元的C端结构域(CT)。CTGF在许多生物过程中发挥重要作用，包括细胞粘附、迁移、增殖、血管生成、骨骼发育和组织创伤修复。监测血清中CTGF水平可为一些病理情况如恶性肿瘤、糖尿病肾病、视网膜病变、纤维化、关节炎、胰腺癌和心血管疾病，提供更详细的见解。

人 Human CCN2 分子靶点信息

分子名：CCN2, cellular communication network factor 2
别称：
- CCN family member 2
- connective tissue growth factor
- CTGF
- HCS24
- hypertrophic chondrocyte-specific protein 24
- IBP-8
- IGF-binding protein 8
- IGFBP-8
- IGFBP8
- insulin-like growth factor-binding protein 8
- MGC102839
- NOV2
基因序列：NCBI_Gene: 1490
蛋白序列：UniProtKB: P29279

人 Human CCN2靶点分子功能（预测）

Enables protein C-terminus binding activity. Involved in positive regulation of MAPK cascade; positive regulation of cell differentiation; and positive regulation of stress fiber assembly. Colocalizes with collagen-containing extracellular matrix. Biomarker of ovarian cancer.