EK9220

Human/Canine/Porcine Insulin ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:INS, Insulin
  • 种属:人 (Human); Canine; Porcine
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:0.35 pmol/L
  • 检测范围:7.81 pmoL/L - 500 pmoL/L
  • 回收率:80% - 130%

在售SKU:70-EK9220-48, 70-EK9220-96

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描述

商品名

Human/Canine/Porcine Insulin ELISA Kit (人/犬/猪胰岛素 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.3% - 4.6%;板间变异系数:3.8% - 4.5%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

0.35 pmol/L

检测范围

7.81 pmoL/L - 500 pmoL/L

回收率

80% - 130%

平均回收率

1

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗Insulin单克隆抗体的96孔聚苯乙烯酶标板
  • 人/犬/猪Insulin冻干标准品
  • Insulin检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人/犬/猪Insulin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Insulin与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Insulin的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human/Canine/Porcine Insulin ELISA Kit (人/犬/猪胰岛素 ELISA试剂盒) - 标准曲线
标准曲线

操作步骤


下载本产品说明书

ELISA操作常见问题

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引用文献


  1. Insulin Resistance Is Associated with Interleukin 1β (Il-1β) in Non-Diabetic Hemodialysis Patients 

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