EK9220

Human/Canine/Porcine Insulin ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：INS, Insulin
种属：人 (Human); Canine; Porcine
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：50 μL
灵敏度：0.35 pmol/L
检测范围：7.81 pmoL/L - 500 pmoL/L
回收率：80% - 130%

在售SKU:70-EK9220-48, 70-EK9220-96

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Size
库存号

描述

商品名	Human/Canine/Porcine Insulin ELISA Kit (人/犬/猪胰岛素 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.3% - 4.6%；板间变异系数：3.8% - 4.5%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	50 μL
灵敏度	0.35 pmol/L
检测范围	7.81 pmoL/L - 500 pmoL/L
回收率	80% - 130%
平均回收率	1
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗Insulin单克隆抗体的96孔聚苯乙烯酶标板人/犬/猪Insulin冻干标准品 Insulin检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人/犬/猪Insulin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的Insulin与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Insulin的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。