EK1351

Human GDNF ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：GDNF, ATF1, ATF2
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：50 μL
灵敏度：1.68 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：80% - 119%

在售SKU:70-EK1351-48, 70-EK1351-96

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库存号

描述

文章目录[隐藏]

分子信息
GDNF 分子靶点信息概述
GDNF 分子靶点综述
- 人 Human GDNF 分子靶点信息
- 人 Human GDNF靶点分子功能（预测）

商品名	Human GDNF ELISA Kit (人胶质细胞源性神经营养因子 (GDNF) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.9% - 3.5%；板间变异系数：3.6%-4.2%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	50 μL
灵敏度	1.68 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	80% - 119%
平均回收率	1.01
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗GDNF单克隆抗体的96孔聚苯乙烯酶标板人GDNF冻干标准品人GDNF检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人GDNF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的GDNF与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中GDNF的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human GDNF ELISA Kit (人胶质细胞源性神经营养因子 (GDNF) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 GDNF, ATF1, ATF2 靶点信息

GDNF 分子靶点信息概述

分子名：GDNF, glial cell derived neurotrophic factor
基因家族：GDNF family ligands
别名：ATF1; ATF2; HFB1-GDNF
全称：astrocyte-derived trophic factor; glial cell line derived neurotrophic factor; glial derived neurotrophic factor

GDNF 分子靶点综述

胶质细胞源性神经营养因子(GDNF)是一种神经营养因子，在中枢神经系统和外周神经系统发育的不同阶段均能促进不同神经元亚群的存活。功能性人GDNF配体是一个二硫键连接的同源二聚体，由两个15kDa的多肽链组成。GDNF由支持细胞、1型星形胶质细胞、雪旺细胞、神经元、松果体细胞和骨骼肌细胞产生，它是一种能促进运动神经元、中脑多巴胺能神经元、浦肯野细胞和交感神经元存活的分泌性蛋白。GDNF对脊髓运动神经元的存活效率比神经营养蛋白高100倍。在帕金森病小鼠模型中发现，GDNF可以改善诸如运动迟缓、僵硬和姿势不稳定等状况。

人 Human GDNF 分子靶点信息

分子名：GDNF, glial cell derived neurotrophic factor
别称：
- astrocyte-derived trophic factor
- ATF
- ATF1
- ATF2
- glial cell line derived neurotrophic factor
- glial cell line-derived neurotrophic factor
- HFB1-GDNF
- hGDNF
- HSCR3
基因序列：NCBI_Gene: 2668
蛋白序列：UniProtKB: P39905

人 Human GDNF靶点分子功能（预测）

Enables protein homodimerization activity. Involved in several processes, including negative regulation of apoptotic process; positive regulation of morphogenesis of an epithelium; and regulation of RNA metabolic process. Acts upstream of or within several processes, including neural crest cell migration; neuron projection development; and positive regulation of cell population proliferation. Located in Golgi apparatus and extracellular region. Implicated in Hirschsprung's disease; Parkinsonism; and epilepsy. Biomarker of acoustic neuroma; autoimmune disease (multiple); neurodegenerative disease (multiple); pancreatic cancer; and vasculitis (multiple).