F11003A05

Anti-Human CD3，PE-Cy7 (Clone:SK7) 人 CD3 流式抗体 (新品)

¥936.00 – ¥2,072.00

说明书

分子靶点：CD3
种属：人 (Human)
标记：PE-Cy7
亚型：Mouse IgG1, κ
克隆号：SK7

在售SKU, 70-AH0030210-20, 70-AH0030210-50, 70-AH0030210-100, AH0030210, 70-F11003A05-25, 70-F11003A05-100, 人, CD3, CD3, CD3, CD3, CD3, CD3, CD3, CD3, CD3, CD3

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Size
库存号

描述

文章目录[隐藏]

结果示例

商品名	Anti-Human CD3 (SK7)，PE-Cy7 流式抗体
靶点	CD3
标记	PE-Cy7
仪器	适用488nm, 532nm, 561nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	SK7
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2 proclin 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色 Anti-Human CD3, PE-Cy7 (F11003A05)和 Anti-Human CD19, FITC (F1101901) 的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Human CD3, PE-Cy7 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300× g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human CD3, PE-Cy7 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。