EK317HS

Rat IL-17A High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

  • 分子靶点:IL17A, IL17, CTLA8
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:0.15 pg/mL
  • 检测范围:1.56 pg/mL - 100 pg/mL
  • 回收率:94% - 117%

在售SKU:70-EK317HS-48, 70-EK317HS-96

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描述

商品名

Rat IL-17A High Sensitivity ELISA Kit (大鼠白细胞介素17A高敏 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.5% - 4.5%;板间变异系数:2.9% - 4.5%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

0.15 pg/mL

检测范围

1.56 pg/mL - 100 pg/mL

回收率

94% - 117%

平均回收率

1.02

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-17AA单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠IL-17AA冻干标准品
  • IL-17AA检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-17A抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-17A与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-17A的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-17A High Sensitivity ELISA Kit (大鼠白细胞介素17A高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 IL17A, IL17, CTLA8 靶点信息

IL17A 分子靶点信息概述

  • 分子名:IL17A, interleukin 17A
  • 基因家族:Interleukins
  • 别名:IL-17A; IL-17
  • 曾用名:CTLA8; IL17
  • 全称:cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)

IL17A 分子靶点综述

IL-17主要由Th17型细胞产生,是目前已发现的30余种白细胞介素中的一员,按序号排在第17位。该家族包括6个成员配体(IL-17A ~ IL-17F)和5个受体(IL-17RA ~ IL-17RD和SEF)。IL-17是一种主要由活化的T细胞产生的致炎细胞因子,可以促进T细胞的激活和刺激上皮细胞、内皮细胞、成纤维细胞产生多种细胞因子,从而导致炎症的产生。

大鼠 Rat Il17a 分子靶点信息

  • 分子名:Il17a, interleukin 17A
  • 别称:
    • CTLA-8
    • cytotoxic T-lymphocyte-associated antigen 8
    • hypothetical protein LOC301289
    • IL-17
    • IL-17A
    • Il17
    • interleukin 17
    • Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
    • interleukin-17A
    • LOC301289
    • similar to Interleukin-17 precursor (IL-17) (Cytotoxic T lymphocyte-associated antigen 8) (CTLA-8)
  • 基因序列:NCBI_Gene: 301289
  • 蛋白序列:

大鼠 Rat Il17a 靶点分子功能(预测)

Predicted to enable protein heterodimerization activity and protein homodimerization activity. Involved in several processes, including cellular response to glucocorticoid stimulus; positive regulation of necrotic cell death; and positive regulation of transcription by RNA polymerase II. Located in cytoplasm and extracellular space. Used to study anti-basement membrane glomerulonephritis; congestive heart failure; myocarditis; pleurisy; and uveitis. Biomarker of several diseases, including acute necrotizing pancreatitis; inflammatory bowel disease (multiple); lipoid nephrosis; lung disease (multiple); and periodontal disease (multiple). Human ortholog(s) of this gene implicated in Mycobacterium avium complex disease; autoimmune disease (multiple); bronchial disease (multiple); macular degeneration; and rhinitis (multiple). Orthologous to human IL17A (interleukin 17A).

操作步骤


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