EK158

Human Granzyme B ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:GZMB, granzyme B, CTLA1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:3.54 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:83% - 104%

在售SKU:70-EK158-48, 70-EK158-96, EK158

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描述

商品名

Human Granzyme B ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

双抗夹心法

精密度

板内变异系数:5.5% - 7.0%;板间变异系数:3.8% - 5.4%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

3.54 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

83% - 104%

平均回收率

0.94

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被羊抗人 Granzyme B捕获抗体的96孔聚苯乙烯酶标板
  • Human Granzyme B冻干标准品
  • Human Granzyme B检测抗体
  • Streptavidin-HRP
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜
  • 标准品稀释液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Human Granzyme B ELISA Kit 检测试剂盒(酶联免疫吸附法) - 标准曲线
标准曲线

GZMB 分子靶点信息概述

  • 分子名:GZMB, granzyme B
  • 基因家族:Granule associated serine proteases of immune defence
  • 别名:CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
  • 曾用名:CTLA1; CSPB
  • 全称:fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

GZMB 分子靶点综述

颗粒酶 B 是丝氨酸蛋白酶颗粒酶家族的一员,主要存在于细胞毒性 T 淋巴细胞(CTL)和自然杀伤细胞(NK)的颗粒中。颗粒酶 B 利用 Caspase 3、Caspase 8 和 Bid 等底物在颗粒介导的细胞凋亡中发挥重要作用。
作为人类基因组中确定的五种颗粒酶(A、B、H、K 和 M)之一,颗粒酶 B (32kDa)的生物学功能及其在健康和疾病方面的用途得到了最广泛的研究。它被合成为前体(247 个残基),一个信号肽(残基 1-18 个),一个前肽(残基 19-20 个)和一个成熟链(残基 21-247 个)。一旦进入颗粒,Granzyme B 被完全加工成成熟链,当前肽 Gly-Glu 被 Cathepsin c 裂解从 N 端去除时,Granzyme B 成为活性蛋白酶。Granzyme B 的蛋白酶活性受到 Serpin B9/蛋白酶抑制剂 9 的严格控制。

GZMB, granzyme B 分子靶点种属信息

人 Human

人 Human GZMB 分子靶点信息

  • 分子名:GZMB, granzyme B
  • 别称:
    • C11
    • cathepsin G-like 1
    • CCPI
    • CGL-1
    • CGL1
    • CSP-B
    • CSPB
    • CTLA-1
    • CTLA1
    • CTSGL1
    • cytotoxic serine protease B
    • cytotoxic T-lymphocyte proteinase 2
    • cytotoxic T-lymphocyte-associated serine esterase 1
    • fragmentin 2
    • fragmentin-2
    • granzyme 2
    • granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
    • granzyme-2
    • HLP
    • human lymphocyte protein
    • lymphocyte protease
    • SECT
    • T-cell serine protease 1-3E
  • 基因序列:NCBI_Gene: 3002
  • 蛋白序列:UniProtKB: P10144

人 Human GZMB靶点分子功能(预测)

Enables serine-type endopeptidase activity. Involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Located in cytolytic granule. Biomarker of COVID-19; bronchiolitis obliterans; and chronic obstructive pulmonary disease.

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