EK158

Human Granzyme B ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：GZMB, granzyme B, CTLA1
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：3.54 pg/mL
检测范围：15.63 pg/mL - 1000 pg/mL
回收率：83% - 104%

在售SKU:70-EK158-48, 70-EK158-96, EK158

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文章目录[隐藏]

GZMB 分子靶点信息概述
GZMB 分子靶点综述
GZMB, granzyme B 分子靶点种属信息
- 人 Human GZMB 分子靶点信息
- 人 Human GZMB靶点分子功能（预测）

商品名	Human Granzyme B ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法	双抗夹心法
精密度	板内变异系数：5.5% - 7.0%；板间变异系数：3.8% - 5.4%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	3.54 pg/mL
检测范围	15.63 pg/mL - 1000 pg/mL
回收率	83% - 104%
平均回收率	0.94
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被羊抗人 Granzyme B捕获抗体的96孔聚苯乙烯酶标板 Human Granzyme B冻干标准品 Human Granzyme B检测抗体 Streptavidin-HRP 检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜标准品稀释液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Human Granzyme B ELISA Kit 检测试剂盒（酶联免疫吸附法） - 标准曲线 — 标准曲线

GZMB 分子靶点信息概述

分子名：GZMB, granzyme B
基因家族：Granule associated serine proteases of immune defence
别名：CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
曾用名：CTLA1; CSPB
全称：fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

GZMB 分子靶点综述

颗粒酶 B 是丝氨酸蛋白酶颗粒酶家族的一员，主要存在于细胞毒性 T 淋巴细胞(CTL)和自然杀伤细胞(NK)的颗粒中。颗粒酶 B 利用 Caspase 3、Caspase 8 和 Bid 等底物在颗粒介导的细胞凋亡中发挥重要作用。
作为人类基因组中确定的五种颗粒酶(A、B、H、K 和 M)之一，颗粒酶 B (32kDa)的生物学功能及其在健康和疾病方面的用途得到了最广泛的研究。它被合成为前体(247 个残基)，一个信号肽(残基 1-18 个)，一个前肽(残基 19-20 个)和一个成熟链(残基 21-247 个)。一旦进入颗粒，Granzyme B 被完全加工成成熟链，当前肽 Gly-Glu 被 Cathepsin c 裂解从 N 端去除时，Granzyme B 成为活性蛋白酶。Granzyme B 的蛋白酶活性受到 Serpin B9/蛋白酶抑制剂 9 的严格控制。

GZMB, granzyme B 分子靶点种属信息

人 Human

人 Human GZMB 分子靶点信息

分子名：GZMB, granzyme B
别称：
- C11
- cathepsin G-like 1
- CCPI
- CGL-1
- CGL1
- CSP-B
- CSPB
- CTLA-1
- CTLA1
- CTSGL1
- cytotoxic serine protease B
- cytotoxic T-lymphocyte proteinase 2
- cytotoxic T-lymphocyte-associated serine esterase 1
- fragmentin 2
- fragmentin-2
- granzyme 2
- granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
- granzyme-2
- HLP
- human lymphocyte protein
- lymphocyte protease
- SECT
- T-cell serine protease 1-3E
基因序列：NCBI_Gene: 3002
蛋白序列：UniProtKB: P10144

人 Human GZMB靶点分子功能（预测）

Enables serine-type endopeptidase activity. Involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Located in cytolytic granule. Biomarker of COVID-19; bronchiolitis obliterans; and chronic obstructive pulmonary disease.