EK293

Mouse EGF ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:EGF, epidermal growth factor
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:0.41 pg/mL
  • 检测范围:3.91 pg/mL - 250 pg/mL
  • 回收率:87% - 117%

在售SKU:70-EK293-48, 70-EK293-96

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描述

商品名

Mouse EGF ELISA Kit (小鼠EGF ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.7% - 3.5%;板间变异系数:2.8% - 4.1%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

0.41 pg/mL

检测范围

3.91 pg/mL - 250 pg/mL

回收率

87% - 117%

平均回收率

1.01

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗Mouse EGF单克隆抗体的96孔聚苯乙烯酶标板
  • Mouse EGF冻干标准品
  • Mouse EGF检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠EGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的EGF与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中EGF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse EGF ELISA Kit (小鼠EGF ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 EGF, epidermal growth factor 靶点信息

EGF 分子靶点信息概述

  • 分子名:EGF, epidermal growth factor
  • 全称:Pro-epidermal growth factor; epidermal growth factor (beta-urogastrone)

EGF 分子靶点综述

表皮生长因子(EGF)是一种通过与其受体EGFR结合,刺激细胞生长、增殖和分化的生长因子。EGF是小分子量多肽,最迟从小鼠下颌下腺纯化得到,随后在很多人的组织中均有发现,包括下颌下腺和腮腺。由饮食中的无机碘调控的唾液EGF在维持奥罗食管和胃组织健全方面具有重要的生理作用。唾液EGF的生理作用包括治愈口腔和胃溃疡、抑制胃酸分泌、刺激DNA合成以及保护粘膜免受腔内有害因素(如胃酸、胆汁酸、胃蛋白酶和胰蛋白酶)和物理、化学和细菌因素破坏。在某些通常与受体的突变和功能异常(如不依赖于EGF水平或与EGF结合的组成性受体信号传导)有关的癌症中,可观察到EGF受体活性增强。

小鼠 Mouse Egf 分子靶点信息

小鼠 Mouse Egf 靶点分子功能(预测)

Enables epidermal growth factor receptor binding activity. Involved in several processes, including negative regulation of cholesterol efflux; positive regulation of ubiquitin-dependent protein catabolic process; and regulation of receptor signaling pathway via JAK-STAT. Acts upstream of or within several processes, including mammary gland alveolus development; positive regulation of cerebellar granule cell precursor proliferation; and positive regulation of protein tyrosine kinase activity. Located in extracellular space and plasma membrane. Is expressed in several structures, including 1st branchial arch; alimentary system; brain; genitourinary system; and liver and biliary system. Human ortholog(s) of this gene implicated in several diseases, including Zollinger-Ellison syndrome; hepatobiliary system cancer (multiple); high grade glioma (multiple); lung non-small cell carcinoma (multiple); and primary hypomagnesemia (multiple). Orthologous to human EGF (epidermal growth factor).

操作步骤


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