EK1312

Human sICAM-3/CD50 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：ICAM3, ICAM-R, CD50
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL(稀释液)
灵敏度：5.09 pg/mL
检测范围：125 pg/mL - 8000 pg/mL
回收率：77% - 112%

在售SKU:70-EK1312-48, 70-EK1312-96

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描述

文章目录[隐藏]

分子信息
ICAM3 分子靶点信息概述
ICAM3 分子靶点综述
- 人 Human ICAM3 分子靶点信息
- 人 Human ICAM3靶点分子功能（预测）

商品名	Human sICAM-3/CD50 ELISA Kit (人可溶性细胞间粘附分子3 (sICAM-3/CD50) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.5% - 3.9%；板间变异系数：1.6% - 6.2%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL(稀释液)
灵敏度	5.09 pg/mL
检测范围	125 pg/mL - 8000 pg/mL
回收率	77% - 112%
平均回收率	0.93
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-17AF单克隆抗体的96孔聚苯乙烯酶标板人IL-17AF冻干标准品 IL-17AF检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sICAM-3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的sICAM-3与固相抗体和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中sICAM-3的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human sICAM-3/CD50 ELISA Kit (人可溶性细胞间粘附分子3 (sICAM-3/CD50) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 ICAM3, ICAM-R, CD50 靶点信息

ICAM3 分子靶点信息概述

分子名：ICAM3, intercellular adhesion molecule 3
基因家族：CD molecules; Immunoglobulin like domain containing; Ig-like cell adhesion molecule family
别名：CDW50; ICAM-R; CD50

ICAM3 分子靶点综述

细胞间粘附分子3 (ICAM-3)，也称为CD50，是免疫球蛋白超家族的成员，可与白细胞整合素LFA-1 (CD11a/CD18)和αd/β2结合。ICAM-3在白细胞和表皮朗格汉斯细胞上表达。ICAM-3是一种由白细胞表达的跨膜糖蛋白，是淋巴细胞功能相关抗原(LFA)-1整合素的反受体。ICAM-3除了作为T细胞与抗原呈递细胞接触的粘附分子外，还可调节LFA-1对ICAM-1的亲和力，并诱导T细胞活化和增殖。ICAM-3在T细胞和抗原呈递细胞的免疫应答启动过程中发挥重要作用。CD50分子在T淋巴细胞功能成熟过程中发挥作用，在T淋巴细胞成熟过程中表达增多。