EK1253

Human Midkine ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：MDK, NEGF2
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：95 μL 1×检测缓冲液和5 μL样本
灵敏度：4.59 pg/mL
检测范围：15.63 pg/mL - 1000 pg/mL
回收率：97% - 118%

在售SKU:70-EK1253-48, 70-EK1253-96

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库存号

描述

文章目录[隐藏]

分子信息
MDK 分子靶点信息概述
MDK 分子靶点综述
- 人 Human MDK 分子靶点信息
- 人 Human MDK靶点分子功能（预测）

商品名	Human Midkine ELISA Kit (人神经轴突生长促进因子2 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.5% - 5.6%；板间变异系数：4.1%-9.0%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	95 μL 1×检测缓冲液和5 μL样本
灵敏度	4.59 pg/mL
检测范围	15.63 pg/mL - 1000 pg/mL
回收率	97% - 118%
平均回收率	1.06
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗Midkine单克隆抗体的96孔聚苯乙烯酶标板人Midkine冻干标准品 Midkine检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Midkine抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的Midkine与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Midkine的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Midkine ELISA Kit (人神经轴突生长促进因子2 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 MDK, NEGF2 靶点信息

MDK 分子靶点信息概述

分子名：MDK, midkine
别名：MK; FLJ27379
曾用名：NEGF2
全称：neurite growth-promoting factor 2

MDK 分子靶点综述

Midkine (MK或MDK)是一种 15 kDa的肝素结合分子，也被称为神经轴突生长促进因子2 (NEGF2)，是一种由MDK基因编码的蛋白。Midkine具有多效性，能发挥细胞增殖、细胞迁移、血管生成和纤维蛋白溶解等作用。在多种癌症类型中如神经母细胞瘤、大肠癌、肝癌、卵巢癌等发现有Midkine的表达。这些恶性肿瘤患者的血清中 Midkine 浓度要比正常人高得多。在某些情况下，这些高浓度的 Midkine 也预示着疾病的预后较差，如神经母细胞瘤、胶质瘤和膀胱癌。Midkine 作为一种低亲和力的受体与细胞表面的核仁素结合。这种结合可以抑制艾滋病毒感染。

人 Human MDK 分子靶点信息

分子名：MDK, midkine
别称：
- amphiregulin-associated protein
- ARAP
- FLJ27379
- midgestation and kidney protein
- midkine (neurite growth-promoting factor 2)
- MK
- NEGF2
- neurite growth-promoting factor 2
- neurite outgrowth-promoting factor 2
- neurite outgrowth-promoting protein
- retinoic acid inducible factor
基因序列：NCBI_Gene: 4192
蛋白序列：UniProtKB: P21741

人 Human MDK靶点分子功能（预测）

Enables heparan sulfate binding activity and heparin binding activity. Involved in several processes, including negative regulation of apoptotic process; positive regulation of cell migration; and regulation of leukocyte cell-cell adhesion. Colocalizes with collagen-containing extracellular matrix. Implicated in brain ischemia. Biomarker of endometriosis.