EK1282

Human Ki-67/MKI67 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：MKI67, Ki-67, MIB-1
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：50 μL
灵敏度：0.35 pg/mL
检测范围：6.25 pg/mL - 400 pg/mL
回收率：85% - 102%

在售SKU:70-EK1282-48, 70-EK1282-96

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库存号

描述

文章目录[隐藏]

分子信息
MKI67 分子靶点信息概述
MKI67 分子靶点综述
- 人 Human MKI67 分子靶点信息
- 人 Human MKI67靶点分子功能（预测）

商品名	Human Ki-67/MKI67 ELISA Kit (人KI-67 MKI67 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：1.9% - 2.5%；板间变异系数：2.4%-3.0%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	50 μL
灵敏度	0.35 pg/mL
检测范围	6.25 pg/mL - 400 pg/mL
回收率	85% - 102%
平均回收率	0.95
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗Ki-67/MKI67单克隆抗体的96孔聚苯乙烯酶标板人Ki-67/MKI67冻干标准品人Ki-67/MKI67检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Ki-67抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的Ki-67与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Ki-67的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Ki-67/MKI67 ELISA Kit (人KI-67 MKI67 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 MKI67, Ki-67, MIB-1 靶点信息

MKI67 分子靶点信息概述

分子名：MKI67, marker of proliferation Ki-67
基因家族：Protein phosphatase 1 regulatory subunits
别名：MIB-1; PPP1R105; Ki-67
全称：protein phosphatase 1, regulatory subunit 105; Molecular Immunology Borstel antibody 1; antigen identified by monoclonal antibody Ki-67

MKI67 分子靶点综述

KI-67也称为Ki-67或MKI67，在人类中是一种由MKI67基因编码的蛋白质。Ki-67是350 - 400kDa的核蛋白，属于染色体有丝分裂相关蛋白的成员。此外，Ki-67与核糖体RNA转录有关，其失活会抑制核糖体RNA的合成。Ki-67与160kDa 的Hklp2相互作用，后者促进中心体分离和纺锤体的两极性。它也直接与NIFK相互作用，并结合UBF，从而在核糖体RNA的合成中发挥作用。在ER阳性乳腺癌患者中，Ki-67代表了一群高增殖性的亚群，使他们在辅助化疗中获益更多。

人 Human MKI67 分子靶点信息

分子名：MKI67, marker of proliferation Ki-67
别称：
- antigen identified by monoclonal antibody Ki-67
- antigen KI-67
- antigen Ki67
- KIA
- MIB-
- MIB-1
- Molecular Immunology Borstel antibody 1
- PPP1R105
- proliferation marker protein Ki-67
- proliferation-related Ki-67 antigen
基因序列：NCBI_Gene: 4288
蛋白序列：UniProtKB: P46013

人 Human MKI67靶点分子功能（预测）

Enables protein C-terminus binding activity. Involved in regulation of chromosome segregation and regulation of mitotic nuclear division. Located in chromosome; nuclear body; and nucleolus. Colocalizes with condensed chromosome. Implicated in Crohn's disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Biomarker of several diseases, including Barrett's esophagus; autoimmune disease of musculoskeletal system (multiple); endocrine gland cancer (multiple); gastrointestinal system cancer (multiple); and interstitial cystitis.