EK135

Human IL-35 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,200.00 – ¥3,800.00

分子靶点：待定
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：76.93 pg/mL
检测范围：0.47 ng/mL - 30 ng/mL
回收率：95% - 119%

在售SKU:70-EK135-48, 70-EK135-96

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Size
库存号

描述

商品名	Human IL-35 ELISA Kit (人白细胞介素35 (IL-35) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.7% - 4.9%；板间变异系数：4.5% - 4.9%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	76.93 pg/mL
检测范围	0.47 ng/mL - 30 ng/mL
回收率	95% - 119%
平均回收率	1.08
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-35单克隆抗体的96孔聚苯乙烯酶标板人IL-35冻干标准品 IL-35检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-12/IL-35 p35抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-35与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-35的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。