EK1307

Human t-Plasminogen Activator (tPA) ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：PLAT, tPA
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL(稀释液)
灵敏度：0.81 pg/mL
检测范围：62.5 pg/mL - 4000 pg/mL
回收率：73% - 113%

在售SKU:70-EK1307-48, 70-EK1307-96

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分子信息
PLAT, tPA 分子靶点信息概述
PLAT, tPA 分子靶点综述
- 人 Human PLAT, tPA 分子靶点信息
- 人 Human PLAT, tPA 靶点分子功能（预测）

商品名	Human t-Plasminogen Activator/tPA ELISA Kit (人组织型纤溶酶原激活剂 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.2% - 5.3%；板间变异系数：2.4% - 7.4%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL(稀释液)
灵敏度	0.81 pg/mL
检测范围	62.5 pg/mL - 4000 pg/mL
回收率	73% - 113%
平均回收率	0.93
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗t-Plasminogen Activator/tPA单克隆抗体的96孔聚苯乙烯酶标板人t-Plasminogen Activator/tPA冻干标准品 t-Plasminogen Activator/tPA检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人tPA抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的tPA与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中tPA的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human t-Plasminogen Activator/tPA ELISA Kit (人组织型纤溶酶原激活剂 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 PLAT, tPA 靶点信息

PLAT, tPA 分子靶点信息概述

分子名：PLAT, plasminogen activator, tissue type
曾用名：plasminogen activator, tissue

PLAT, tPA 分子靶点综述

组织型纤溶酶原激活剂(tPA)是一种丝氨酸蛋白酶，它将纤溶酶原转化为活性丝氨酸蛋白酶纤溶酶，丝氨酸蛋白酶纤溶酶是去除纤维蛋白沉积的主要酶。tPA 的生物学效应包括血块降解、血管重塑、突触可塑性和创伤后大脑的神经变性。通过低密度脂蛋白受体相关蛋白-1 介导的内吞作用，tPA 也能够迅速从细胞外和血管内清除。tPA 的表达或活性增加与过度出血有关，而 tPA 活性降低与血栓形成和栓塞形成有关。在大脑中，tPA 在神经元、星形细胞、小胶质细胞和血管实质内皮细胞中表达。在中风、缺氧、兴奋性毒性创伤和应激诱导的认知功能下降后，大脑中 tPA 的表达发生了变化。

人 Human PLAT, tPA 分子靶点信息

分子名：PLAT, plasminogen activator, tissue type
别称：
- alteplase
- DKFZp686I03148
- plasminogen/activator kringle
- reteplase
- T-PA
- t-plasminogen activator
- tissue plasminogen activator (t-PA)
- tissue-type plasminogen activator
- TPA
基因序列：NCBI_Gene: 5327
蛋白序列：UniProtKB: P00750

人 Human PLAT, tPA 靶点分子功能（预测）

Enables phosphoprotein binding activity; serine-type endopeptidase activity; and signaling receptor binding activity. Involved in negative regulation of proteolysis and plasminogen activation. Located in cell surface; cytoplasm; and serine protease inhibitor complex. Implicated in several diseases, including antiphospholipid syndrome; diabetic angiopathy; factor VIII deficiency; osteonecrosis; and thrombophilia due to decreased release of PLAT. Biomarker of several diseases, including Henoch-Schoenlein purpura; autoimmune thrombocytopenic purpura; blood coagulation disease (multiple); diabetes mellitus (multiple); and hemolytic-uremic syndrome.