EK1252

Human Bcl-2 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：BCL2, Bcl-2
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：80 μL 1×检测缓冲液和20 μL样本
灵敏度：50.25 pg/mL
检测范围：0.47 ng/mL - 30 ng/mL
回收率：90% - 110%

在售SKU:70-EK1252-48, 70-EK1252-96

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文章目录[隐藏]

分子信息
BCL2 分子靶点信息概述
BCL2 分子靶点综述
- 人 Human BCL2 分子靶点信息
- 人 Human BCL2靶点分子功能（预测）

商品名	Human Bcl-2 ELISA Kit (人B细胞淋巴瘤2 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.0% - 4.9%；板间变异系数：2.4% - 4.7%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	80 μL 1×检测缓冲液和20 μL样本
灵敏度	50.25 pg/mL
检测范围	0.47 ng/mL - 30 ng/mL
回收率	90% - 110%
平均回收率	0.99
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗Bcl-2单克隆抗体的96孔聚苯乙烯酶标板人Bcl-2冻干标准品 Bcl-2检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Bcl-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的Bcl-2与固相抗体结合和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中Bcl-2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Bcl-2 ELISA Kit (人B细胞淋巴瘤2 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 BCL2, Bcl-2 靶点信息

BCL2 分子靶点信息概述

分子名：BCL2, BCL2 apoptosis regulator
基因家族：Protein phosphatase 1 regulatory subunits; BCL2 family
别名：Bcl-2; PPP1R50
全称：protein phosphatase 1, regulatory subunit 50; B-cell CLL/lymphoma 2; BCL2, apoptosis regulator

BCL2 分子靶点综述

B细胞淋巴瘤2 (Bcl-2)，是Bcl-2家族调节蛋白中的创始成员，可通过诱导凋亡(促凋亡)或者抑制凋亡(抗凋亡)来调节细胞凋亡。Bcl-2已明确是一种重要的抗凋亡蛋白，因此被归类为是一种致癌基因。Bcl-2调节线粒体动力学，并参与线粒体融合与分裂的调控。另外，在胰岛β细胞中，Bcl-2和Bcl-XL参与控制代谢活性和胰岛素分泌。 Bcl-2基因损伤会引发许多癌症，包括黑色素瘤、乳腺癌、前列腺癌、慢性淋巴细胞白血病和肺癌，还可能与精神分裂症和自身免疫性疾病有关。Bcl-2也是癌症治疗抵抗的一个原因。

人 Human BCL2 分子靶点信息

分子名：BCL2, BCL2 apoptosis regulator
别称：
- apoptosis regulator Bcl-2
- B-cell CLL/lymphoma 2
- b-cell leukemia/lymphoma 2
- Bcl-2
- Bcl-2a
- Bcl-2?
- BCL2, apoptosis regulator
- PPP1R50
- protein phosphatase 1, regulatory subunit 50
基因序列：NCBI_Gene: 596
蛋白序列：UniProtKB: P10415

人 Human BCL2靶点分子功能（预测）

Enables several functions, including BH3 domain binding activity; enzyme binding activity; and protein heterodimerization activity. Involved in several processes, including B cell receptor signaling pathway; negative regulation of apoptotic process; and positive regulation of B cell proliferation. Acts upstream of or within several processes, including B cell proliferation; apoptotic signaling pathway; and negative regulation of apoptotic process. Located in endoplasmic reticulum; mitochondrial outer membrane; and nucleus. Part of pore complex. Implicated in several diseases, including carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; prostate cancer; and urinary bladder cancer. Biomarker of several diseases, including cervix uteri carcinoma in situ; ductal carcinoma in situ; gastrointestinal system cancer (multiple); hematologic cancer (multiple); and urinary system cancer (multiple).