F11011b03

Anti-Human CD11b, APC (Clone: LT11)流式抗体 (新品)

X

¥680.00¥1,504.00

在售SKU, 70-AH011B05-20, 70-AH011B05-50, 70-AH011B05-100, AH011B05, 70-F11011B03-25, 70-F11011B03-100

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描述

商品名

Anti-Human CD11b, APC (Clone: LT11)流式抗体

靶点

CD11B, MAC-1, ITGAM, CR3A

标记

APC

仪器

适用633nm激光器的流式细胞仪

亚型

Mouse IgG1

克隆号

LT11

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD11b, APC (Clone: LT11)流式抗体 - 结果示例图片Anti-Human CD11b, APC (Clone: LT11)流式抗体 - 结果示例图片
结果示例

图 - 人外周血染色左图为Mouse IgG1 Istype control,APC,右图为 Anti-Human CD11b, APC(Clone: LT11)(F11011b03/3) 的结果。以淋巴细胞、粒细胞和单核细胞设门进行分析。

分子信息

概述

ITGAM 分子靶点信息概述

  • 分子名:ITGAM, integrin subunit alpha M
  • 基因家族:CD molecules; Integrin alpha subunits; Complement system regulators and receptors
  • 别名:MAC-1; CD11b
  • 曾用名:CR3A; CD11B
  • 全称:complement component 3 receptor 3 subunit; macrophage-1 antigen alpha subunit; integrin, alpha M (complement component receptor 3, alpha; also known as CD11b (p170), macrophage antigen alpha polypeptide); integrin, alpha M (complement component 3 receptor 3 subunit)

ITGAM 分子靶点综述

CD11b与CD18非共价结合,形成αM-β2整合素(Mac-1),与CD54(ICAM-1)、C3bi和纤维蛋白原结合。Mac-1表达于巨噬细胞、NK细胞、粒细胞、小胶质细胞、活化的淋巴细胞和小鼠B-1细胞。CD11b与其配体结合,参与粘附细胞的互相作用。此抗体也可交叉识别人CD11b,可检测人外周血中单核细胞、粒细胞和某一群NK细胞上的该抗原。

操作步骤


下载本产品说明书

使用方法

全血样本:取100 μl 抗凝血,加入5 μl Anti-Human CD11b,APC 和其他抗体,震荡混匀,于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300×g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号S1001)重悬,加入5 μl Anti-Human CD11b,APC 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心5 - 10分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

查看更多FCM流式检测相关问题

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