F1122303

Anti-Human CD223, APC (Clone: OTI5D8)检测试剂

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¥1,360.00¥3,168.00

  • 分子靶点:CD223, LAG3
  • 种属:人 (Human)
  • 标记:APC
  • 亚型:Mouse IgG1
  • 克隆号:OTI5D8

在售SKU, F1122303, 70-F1122303-100, 70-F1122303-25

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描述

商品名

Anti-Human CD223, APC (Clone: OTI5D8)检测试剂

靶点

CD223, LAG3

标记

APC

仪器

适用633nm激光器的流式细胞仪

亚型

Mouse IgG1

克隆号

OTI5D8

推荐应用

流式检测

用量

5 μL/Test

纯化

亲和层析

储存液

含 0.2% BSA 和0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD223, APC (Clone: OTI5D8)流式抗体 检测试剂 - 结果示例图片Anti-Human CD223, APC (Clone: OTI5D8)流式抗体 检测试剂 - 结果示例图片
结果示例

图 - CD3、CD28抗体诱导的人PBMC染色左图为Mouse IgG1 Isotype control, APC和Anti-Human CD4, FITC的结果,右图为Anti-Human CD223, APC(F1122303)和Anti-Human CD4, FITC的结果,以全部细胞设门。

分子信息

概述人 CD223, LAG3 靶点信息

LAG3 分子靶点信息概述

  • 分子名:LAG3, lymphocyte activating 3
  • 基因家族:CD molecules; Immunoglobulin like domain containing
  • 别名:CD223
  • 全称:lymphocyte-activation gene 3

LAG3 分子靶点综述

CD223,即淋巴细胞活化基因3(LAG-3),其结合MHC-II比CD4具有更高亲和力,所以被认为与T细胞活化的负调控以及稳态增殖有关。LAG-3有报道说在活化的T细胞(包括调节性T细胞)以及NK细胞上表达。CD8+T细胞上通常比CD4+T细胞表达更多的LAG-3。 LAG-3与CD49b的共表达被推荐用于鉴定人和小鼠的Tr1细胞。

人 Human LAG3 分子靶点信息

  • 分子名:LAG3, lymphocyte activating 3
  • 别称:
    • CD223
    • lymphocyte activation gene 3 protein
    • lymphocyte-activation gene 3
  • 基因序列:NCBI_Gene: 3902
  • 蛋白序列:UniProtKB: P18627

人 Human LAG3靶点分子功能(预测)

Enables MHC class II protein binding activity. Predicted to be involved in several processes, including cell surface receptor signaling pathway; negative regulation of regulatory T cell differentiation; and plasmacytoid dendritic cell activation. Predicted to act upstream of or within negative regulation of T cell activation; negative regulation of interleukin-2 production; and positive regulation of natural killer cell mediated cytotoxicity. Predicted to be located in extracellular region.

操作步骤


下载本产品说明书

使用方法

全血样本:取100 μl 抗凝血,加入5 μl Anti-Human CD223, APC 和其他抗体,震荡混匀,于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300×g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号S1001)重悬,加入5 μl Anti-Human CD223, APC 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

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