F1101905

Anti-Human CD19, PE-Cy7 (Clone:SJ25C1) 流式抗体

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¥1,152.00¥2,560.00

  • 分子靶点:CD19
  • 种属:人 (Human)
  • 标记:PE-Cy7
  • 亚型:Mouse IgG1, κ
  • 克隆号:SJ25C1

在售SKU:70-F1101905-25, 70-F1101905-100

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描述

商品名

Anti-Human CD19, PE-Cy7 (Clone:SJ25C1) 流式抗体

靶点

CD19

标记

PE-Cy7

仪器

适用488nm, 532nm, 561nm激光器的流式细胞仪

亚型

Mouse IgG1, κ

克隆号

SJ25C1

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.02% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD19, PE-Cy7 (Clone:SJ25C1) 流式抗体 - 结果示例图片
结果示例

图 - 人外周血染色 Anti-Human CD19, PE-Cy7 (F1101905)的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述小鼠 CD19 靶点信息

CD19 分子靶点信息概述

  • 分子名:CD19, CD19 molecule
  • 基因家族:CD molecules; Immunoglobulin like domain containing; Minor histocompatibility antigens
  • 全称:CD19 antigen

CD19 分子靶点综述

CD19 是 95kDa 的跨膜糖蛋白,属于免疫球蛋白超家族的成员,除了浆细胞,表达于B细胞的各个发育阶段。 CD19与CD21、CD81和MHC II类分子形成复合物,通过与B细胞受体作用,起始成熟B细胞的活化。 CD19可促进B细胞增殖、发育和活化,以及记忆性B细胞的成熟。在CD19缺失的小鼠中,骨髓和外周的B细胞生成和成熟受到影响。

小鼠 Mouse Cd19 分子靶点信息

小鼠 Mouse Cd19 靶点分子功能(预测)

Involved in several processes, including B-1 B cell differentiation; positive regulation of phosphatidylinositol 3-kinase activity; and positive regulation of release of sequestered calcium ion into cytosol. Acts upstream of or within B cell receptor signaling pathway. Located in external side of plasma membrane. Is integral component of plasma membrane. Is expressed in liver and spleen. Human ortholog(s) of this gene implicated in common variable immunodeficiency. Orthologous to human CD19 (CD19 molecule).

操作步骤


下载本产品说明书

使用方法

全血样本:取100 μl 抗凝血,加入5 μl Anti-Human CD19, PE-Cy7 和其他抗体,震荡混匀,于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300× g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号S1001)重悬,加入5 μl Anti-Human CD19, PE-Cy7 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心 5 - 10分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

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