F1101905

Anti-Human CD19, PE-Cy7 (Clone:SJ25C1) 流式抗体

¥1,152.00 – ¥2,560.00

说明书

分子靶点：CD19
种属：人 (Human)
标记：PE-Cy7
亚型：Mouse IgG1, κ
克隆号：SJ25C1

在售SKU:70-F1101905-25, 70-F1101905-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
CD19 分子靶点信息概述
CD19 分子靶点综述
- 小鼠 Mouse Cd19 分子靶点信息
- 小鼠 Mouse Cd19 靶点分子功能（预测）

商品名	Anti-Human CD19, PE-Cy7 (Clone:SJ25C1) 流式抗体
靶点	CD19
标记	PE-Cy7
仪器	适用488nm, 532nm, 561nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	SJ25C1
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.02% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色 Anti-Human CD19, PE-Cy7 (F1101905)的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述小鼠 CD19 靶点信息

CD19 分子靶点信息概述

分子名：CD19, CD19 molecule
基因家族：CD molecules; Immunoglobulin like domain containing; Minor histocompatibility antigens
全称：CD19 antigen

CD19 分子靶点综述

CD19 是 95kDa 的跨膜糖蛋白，属于免疫球蛋白超家族的成员，除了浆细胞，表达于B细胞的各个发育阶段。 CD19与CD21、CD81和MHC II类分子形成复合物，通过与B细胞受体作用，起始成熟B细胞的活化。 CD19可促进B细胞增殖、发育和活化，以及记忆性B细胞的成熟。在CD19缺失的小鼠中，骨髓和外周的B细胞生成和成熟受到影响。

小鼠 Mouse Cd19 分子靶点信息

分子名：Cd19, CD19 antigen
别称：
- AW495831
- expressed sequence AW495831
基因序列：NCBI_Gene: 12478
蛋白序列：

小鼠 Mouse Cd19 靶点分子功能（预测）

Involved in several processes, including B-1 B cell differentiation; positive regulation of phosphatidylinositol 3-kinase activity; and positive regulation of release of sequestered calcium ion into cytosol. Acts upstream of or within B cell receptor signaling pathway. Located in external side of plasma membrane. Is integral component of plasma membrane. Is expressed in liver and spleen. Human ortholog(s) of this gene implicated in common variable immunodeficiency. Orthologous to human CD19 (CD19 molecule).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

全血样本：取100 μl 抗凝血，加入5 μl Anti-Human CD19, PE-Cy7 和其他抗体，震荡混匀，于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300× g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号S1001)重悬，加入5 μl Anti-Human CD19, PE-Cy7 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。