EK8100

Cortisol Competitive ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,900.00 – ¥3,200.00

分子靶点：待定
种属：人 (Human); 小鼠 (Mouse); 大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL
灵敏度：66.43 pg/mL
检测范围：0.12 ng/mL - 15 ng/mL
回收率：80% - 130%

在售SKU:70-EK8100-48, 70-EK8100-96

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Size
库存号

描述

商品名	Cortisol Competitive ELISA Kit (皮质醇竞争法 ELISA试剂盒)
检测方法	竞争法
精密度	板内变异系数：4.3% - 4.6%；板间变异系数：6.4% - 7.8%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL
灵敏度	66.43 pg/mL
检测范围	0.12 ng/mL - 15 ng/mL
回收率	80% - 130%
平均回收率	1.18
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗Competitive单克隆抗体的96孔聚苯乙烯酶标板皮质醇Competitive冻干标准品 Competitive检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用固相竞争酶联免疫吸附检测技术。兔抗小鼠抗体预包被在高亲和力的酶标板上。皮质醇特异性的单克隆抗体加入至孔中，经过孵育，与固相抗体结合。洗涤后，加入辣根过氧化物酶标记的皮质醇和未标记的皮质醇或样本，竞争结合单克隆抗体上有限的结合位点。在洗涤去除未结合的物质后，加入显色底物，颜色反应的深浅与皮质醇的浓度成反比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。本试剂盒已验证人、小鼠和大鼠血清样本，但其它物种的皮质醇预计也可被检测。