EK301BHS

Rat IL-1β High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)

X

¥2,000.00¥3,400.00

  • 分子靶点:IL1B, IL1-β, IL1F2
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:20 μL;细胞培养上清:100 μL
  • 灵敏度:1.56 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:90% - 120%

在售SKU:70-EK301BHS-48, 70-EK301BHS-96,70-EK301BHS/2-48, 70-EK301BHS/2-96

¥<\/span>2,000.00<\/bdi><\/span><\/span>","sku":"70-EK301BHS\/2-48","variation_description":"","variation_id":7951,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"96T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":3400,"display_regular_price":3400,"image":{"title":"ELISA-1.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":5986,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>3,400.00<\/bdi><\/span><\/span>","sku":"70-EK301BHS\/2-96","variation_description":"","variation_id":7952,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

描述

商品名

Rat IL-1β High Sensitivity ELISA Kit (大鼠白细胞介素1β高敏 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.9% - 6.3%;板间变异系数:3.7% - 8.6%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:20 μL;细胞培养上清:100 μL

灵敏度

1.56 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

90% - 120%

平均回收率

1.03

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-1β单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠IL-1β冻干标准品
  • IL-1β检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠 IL- 1β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的 IL- 1β与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入 Streptavidin-HRP。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中 IL- 1β的浓度成正比。加入终止液终止反应,在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Rat IL-1β High Sensitivity ELISA Kit (大鼠白细胞介素1β高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 IL1B, IL1-β, IL1F2 靶点信息

IL1B 分子靶点信息概述

  • 分子名:IL1B, interleukin 1 beta
  • 基因家族:Interleukins
  • 别名:IL1F2; IL-1B; IL1-BETA

IL1B 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽,分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体,通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者,参与许多细胞活性,包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族,它们由生物活化的单核细胞所衍生,参与炎症反应和免疫应答。IL-1β表达升高会产生各种不同的自身炎症综合征,最显著的是单基因条件称为CAPS,是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。本试剂盒用来检测人IL-1β的成熟形式和未裂解的前体形式。

大鼠 Rat Il1b 分子靶点信息

大鼠 Rat Il1b 靶点分子功能(预测)

Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

操作步骤


下载本产品说明书

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献


评价

目前还没有评价

成为第一个“Rat IL-1β High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注