EK301BHS

Rat IL-1β High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：IL1B, IL1-β, IL1F2
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：血清血浆：20 μL；细胞培养上清：100 μL
灵敏度：1.56 pg/mL
检测范围：7.81 pg/mL - 500 pg/mL
回收率：90% - 120%

在售SKU:70-EK301BHS-48, 70-EK301BHS-96,70-EK301BHS/2-48, 70-EK301BHS/2-96

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Size
库存号

描述

文章目录[隐藏]

分子信息
IL1B 分子靶点信息概述
IL1B 分子靶点综述
- 大鼠 Rat Il1b 分子靶点信息
- 大鼠 Rat Il1b 靶点分子功能（预测）

商品名	Rat IL-1β High Sensitivity ELISA Kit (大鼠白细胞介素1β高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.9% - 6.3%；板间变异系数：3.7% - 8.6%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	血清血浆：20 μL；细胞培养上清：100 μL
灵敏度	1.56 pg/mL
检测范围	7.81 pg/mL - 500 pg/mL
回收率	90% - 120%
平均回收率	1.03
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-1β单克隆抗体的96孔聚苯乙烯酶标板大鼠IL-1β冻干标准品 IL-1β检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素信号增强剂浓缩液信号增强剂稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠 IL- 1β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的 IL- 1β与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入 Streptavidin-HRP。洗涤后，加入显色底物 TMB，避光显色。颜色反应的深浅与样本中 IL- 1β的浓度成正比。加入终止液终止反应，在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Rat IL-1β High Sensitivity ELISA Kit (大鼠白细胞介素1β高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述大鼠 IL1B, IL1-β, IL1F2 靶点信息

IL1B 分子靶点信息概述

分子名：IL1B, interleukin 1 beta
基因家族：Interleukins
别名：IL1F2; IL-1B; IL1-BETA

IL1B 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽，分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体，通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者，参与许多细胞活性，包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族，它们由生物活化的单核细胞所衍生，参与炎症反应和免疫应答。IL-1β表达升高会产生各种不同的自身炎症综合征，最显著的是单基因条件称为CAPS，是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。本试剂盒用来检测人IL-1β的成熟形式和未裂解的前体形式。

大鼠 Rat Il1b 分子靶点信息

分子名：Il1b, interleukin 1 beta
别称：
- IL-1 beta
- IL-1F2
- interleukin-1 beta
基因序列：NCBI_Gene: 24494
蛋白序列：
- UniprotKB: Q5BKB0
- UniprotKB: Q63264

大鼠 Rat Il1b 靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).