EK380HS

Rat IFN-γ High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

  • 分子靶点:IFNG, IFN-γ
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:0.26 pg/mL
  • 检测范围:3.13 pg/mL - 200 pg/mL
  • 回收率:73% - 137%

在售SKU:70-EK380HS-48, 70-EK380HS-96

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描述

商品名

Rat IFN-γ High Sensitivity ELISA Kit (大鼠γ干扰素高敏 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:3.7% - 4.4%;板间变异系数:2.4% - 8.5%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

0.26 pg/mL

检测范围

3.13 pg/mL - 200 pg/mL

回收率

73% - 137%

平均回收率

0.93

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IFN-γA单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠IFN-γA冻干标准品
  • IFN-γA检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IFN-γ与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IFN-γ High Sensitivity ELISA Kit (大鼠γ干扰素高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 IFNG, IFN-γ 靶点信息

IFNG 分子靶点信息概述

  • 分子名:IFNG, interferon gamma
  • 基因家族:Interferons

IFNG 分子靶点综述

IFN-γ主要由Th1型细胞产生,属于II型细胞因子中干扰素家族中的一员,是一类分泌型糖蛋白,由143个氨基酸组成,相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生,具有调节机体免疫状态,介导细胞免疫,加强受体介导的吞噬功能,增强巨噬细胞和中性粒细胞的杀伤活性,具有抗病毒感染及抗肿瘤等生物学活性。

大鼠 Rat Ifng 分子靶点信息

大鼠 Rat Ifng 靶点分子功能(预测)

Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cell population proliferation; positive regulation of NMDA glutamate receptor activity; and positive regulation of macromolecule metabolic process. Located in several cellular components, including extracellular space; neuron projection; and perikaryon. Used to study several diseases, including Chagas disease; allergic conjunctivitis; allergic rhinitis; renal fibrosis; and ureteral obstruction. Biomarker of several diseases, including abdominal aortic aneurysm; autoimmune thyroiditis; pulpitis; tongue squamous cell carcinoma; and visual epilepsy. Human ortholog(s) of this gene implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).

操作步骤


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