EK380HS

Rat IFN-γ High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：IFNG, IFN-γ
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.26 pg/mL
检测范围：3.13 pg/mL - 200 pg/mL
回收率：73% - 137%

在售SKU:70-EK380HS-48, 70-EK380HS-96

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库存号

描述

文章目录[隐藏]

分子信息
IFNG 分子靶点信息概述
IFNG 分子靶点综述
- 大鼠 Rat Ifng 分子靶点信息
- 大鼠 Rat Ifng 靶点分子功能（预测）

商品名	Rat IFN-γ High Sensitivity ELISA Kit (大鼠γ干扰素高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.7% - 4.4%；板间变异系数：2.4% - 8.5%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.26 pg/mL
检测范围	3.13 pg/mL - 200 pg/mL
回收率	73% - 137%
平均回收率	0.93
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IFN-γA单克隆抗体的96孔聚苯乙烯酶标板大鼠IFN-γA冻干标准品 IFN-γA检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IFN-γ与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IFN-γ High Sensitivity ELISA Kit (大鼠γ干扰素高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述大鼠 IFNG, IFN-γ 靶点信息

IFNG 分子靶点信息概述

分子名：IFNG, interferon gamma
基因家族：Interferons

IFNG 分子靶点综述

IFN-γ主要由Th1型细胞产生，属于II型细胞因子中干扰素家族中的一员，是一类分泌型糖蛋白，由143个氨基酸组成，相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生，具有调节机体免疫状态，介导细胞免疫，加强受体介导的吞噬功能，增强巨噬细胞和中性粒细胞的杀伤活性，具有抗病毒感染及抗肿瘤等生物学活性。

大鼠 Rat Ifng 分子靶点信息

分子名：Ifng, interferon gamma
别称：
- If2f
- IFN-gamma
- IFNG2
- interferon 2f
基因序列：NCBI_Gene: 25712
蛋白序列：
- UniprotKB: A0A7R8C3J6
- UniprotKB: P01581

大鼠 Rat Ifng 靶点分子功能（预测）

Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cell population proliferation; positive regulation of NMDA glutamate receptor activity; and positive regulation of macromolecule metabolic process. Located in several cellular components, including extracellular space; neuron projection; and perikaryon. Used to study several diseases, including Chagas disease; allergic conjunctivitis; allergic rhinitis; renal fibrosis; and ureteral obstruction. Biomarker of several diseases, including abdominal aortic aneurysm; autoimmune thyroiditis; pulpitis; tongue squamous cell carcinoma; and visual epilepsy. Human ortholog(s) of this gene implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).