EK2208

Mouse TRANCE/TNFSF11/RANKL ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：TNFSF11, RANKL, CD254
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：4.23 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：83% - 116%

在售SKU:70-EK2208-48, 70-EK2208-96

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描述

文章目录[隐藏]

分子信息
TNFSF11 分子靶点信息概述
TNFSF11 分子靶点综述
- 小鼠 Mouse Tnfsf11 分子靶点信息
- 小鼠 Mouse Tnfsf11 靶点分子功能（预测）

商品名	Mouse TRANCE/TNFSF11/RANKL ELISA Kit (小鼠肿瘤坏死因子相关激活诱导因子/肿瘤坏死因子配体超家族成员11/核因子κB受体活化因子配基 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：6.0%-8.9%；板间变异系数：5.8% - 8.6%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	4.23 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	83% - 116%
平均回收率	1
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗TRANCE单克隆抗体的96孔聚苯乙烯酶标板小鼠TRANCE冻干标准品 TRANCE检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠 TRANCE 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的 TRANCE 与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物 TMB，避光显色。颜色反应的深浅与样本中 TRANCE 的浓度成正比。加入终止液终止反应，在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Mouse TRANCE/TNFSF11/RANKL ELISA Kit (小鼠肿瘤坏死因子相关激活诱导因子/肿瘤坏死因子配体超家族成员11/核因子κB受体活化因子配基 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 TNFSF11, RANKL, CD254 靶点信息

TNFSF11 分子靶点信息概述

分子名：TNFSF11, TNF superfamily member 11
基因家族：CD molecules; Tumor necrosis factor superfamily
别名：TRANCE; RANKL; OPGL; ODF; CD254
全称：tumor necrosis factor (ligand) superfamily, member 11

TNFSF11 分子靶点综述

肿瘤坏死因子相关激活诱导因子(TRANCE)，又名肿瘤坏死因子配体超家族成员11(TNFSF11)、核因子κB受体活化因子配基(RANKL)，是TNF超家族成员。成骨细胞、CD4+和CD8+T细胞、巨核细胞、支气管和肠道上皮细胞等都可表达TRANCE。TRANCE在免疫应答和骨形态发生中起核心作用。在骨形成和重塑过程中，成骨细胞表达的TRANCE与M-CSF一起诱导破骨细胞形成和活化，导致骨吸收，进而导致随后的骨沉积和重塑。TRANCE同样在病理性骨质疏松中起作用。活化的淋巴细胞和成骨细胞不恰当表达TRANCE就会导致关节和骨头的过度吸收与沉积。过量的TRANCE与许多退行性骨疾病，如类风湿性关节炎、银屑病性关节炎有关。

小鼠 Mouse Tnfsf11 分子靶点信息

分子名：Tnfsf11, tumor necrosis factor (ligand) superfamily, member 11
别称：
- Ly109l
- ODF
- OPGL
- osteoclast differentiation factor
- RANKL
- TNF-related activation-induced cytokine
- Trance
基因序列：NCBI_Gene: 21943
蛋白序列：
- UniprotKB: O35235
- UniprotKB: Q059M3

小鼠 Mouse Tnfsf11 靶点分子功能（预测）

Enables identical protein binding activity and tumor necrosis factor receptor superfamily binding activity. Involved in several processes, including intracellular signal transduction; positive regulation of secretion by cell; and tumor necrosis factor-mediated signaling pathway. Acts upstream of or within several processes, including animal organ development; cellular response to cytokine stimulus; and positive regulation of intracellular signal transduction. Predicted to be located in cytoplasm; extracellular space; and plasma membrane. Predicted to be integral component of membrane. Is expressed in genitourinary system; hemolymphoid system; hindlimb long bone; and lymphatic system. Used to study autosomal recessive osteopetrosis 2 and osteoporosis. Human ortholog(s) of this gene implicated in autosomal recessive osteopetrosis 2 and osteoporosis. Orthologous to human TNFSF11 (TNF superfamily member 11).