EK2161

Mouse TRAIL/TNFSF10 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：TNFSF10, TRAIL, TL2, CD253
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.52 pg/mL
检测范围：15.63 pg/mL - 1000 pg/mL
回收率：78% - 95%

在售SKU:70-EK2161-48, 70-EK2161-96

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描述

文章目录[隐藏]

分子信息
TNFSF10 分子靶点信息概述
TNFSF10 分子靶点综述
- 小鼠 Mouse Tnfsf10 分子靶点信息
- 小鼠 Mouse Tnfsf10 靶点分子功能（预测）

商品名	Mouse TRAIL/TNFSF10 ELISA Kit (小鼠肿瘤坏死因子相关的凋亡诱导配体 (TRAIL) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：1.8% - 3.2%；板间变异系数：3.7%-4.6%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.52 pg/mL
检测范围	15.63 pg/mL - 1000 pg/mL
回收率	78% - 95%
平均回收率	0.85
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗TRAIL单克隆抗体的96孔聚苯乙烯酶标板小鼠TRAIL冻干标准品小鼠TRAIL检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠TRAIL抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的TRAIL与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TRAIL的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse TRAIL/TNFSF10 ELISA Kit (小鼠肿瘤坏死因子相关的凋亡诱导配体 (TRAIL) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 TNFSF10, TRAIL, TL2, CD253 靶点信息

TNFSF10 分子靶点信息概述

分子名：TNFSF10, TNF superfamily member 10
基因家族：CD molecules; Tumor necrosis factor superfamily
别名：TRAIL; Apo-2L; TL2; CD253
全称：tumor necrosis factor (ligand) superfamily, member 10

TNFSF10 分子靶点综述

肿瘤坏死因子相关的凋亡诱导配体(TRAIL)也称为CD253或TNFSF10，可由大部分正常组织细胞产生并分泌。TRAIL与死亡受体DR4(TRAIL-RI)和DR5(TRAIL-RII)结合。凋亡的过程依赖于caspase-8。Caspase-8活化下游的caspase，包括procaspase-3、6和7，使特异性的激酶活化。TRAIL也能与不含胞浆区的受体DcR1和含有截短的死亡结构域的受体DcR2结合。DcR1可作为TRAIL中和受体。DcR2的胞浆区具有功能，可活化NF-κB。因此在表达DcR2的细胞中，TRAIL的结合会活化NF-κB，使拮抗死亡信号通路和/或促进炎症的基因发生转录。

小鼠 Mouse Tnfsf10 分子靶点信息

分子名：Tnfsf10, tumor necrosis factor (ligand) superfamily, member 10
别称：
- A330042I21Rik
- AI448571
- APO-2L
- expressed sequence AI448571
- RIKEN cDNA A330042I21 gene
- TNF-related apoptosis inducing ligand
- Trail
基因序列：NCBI_Gene: 22035
蛋白序列：

小鼠 Mouse Tnfsf10 靶点分子功能（预测）

Enables TRAIL binding activity and tumor necrosis factor receptor superfamily binding activity. Acts upstream of or within positive regulation of extrinsic apoptotic signaling pathway. Predicted to be located in extracellular space and plasma membrane. Predicted to be integral component of membrane. Is expressed in central nervous system; genitourinary system; gut; and retina. Human ortholog(s) of this gene implicated in arteriosclerosis and small cell carcinoma. Orthologous to human TNFSF10 (TNF superfamily member 10).