EK2137

Mouse PDGF-BB ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：PDGFB
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL(稀释液)
灵敏度：1.05 pg/mL
检测范围：23.44 pg/mL - 1500 pg/mL
回收率：92% - 122%

在售SKU:70-EK2137-48, 70-EK2137-96

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库存号

描述

文章目录[隐藏]

分子信息
PDGFB 分子靶点信息概述
PDGFB 分子靶点综述
- 小鼠 Mouse Pdgfb 分子靶点信息
- 小鼠 Mouse Pdgfb 靶点分子功能（预测）

商品名	Mouse PDGF-BB ELISA Kit (小鼠血小板衍生因子BB (PDGF-BB) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.5% - 5.0%；板间变异系数：3.3%-5.1%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL(稀释液)
灵敏度	1.05 pg/mL
检测范围	23.44 pg/mL - 1500 pg/mL
回收率	92% - 122%
平均回收率	1.08
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗PDGF-BB单克隆抗体的96孔聚苯乙烯酶标板小鼠PDGF-BB冻干标准品小鼠PDGF-BB检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠PDGF-BB抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的PDGF-BB与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中PDGF-BB的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse PDGF-BB ELISA Kit (小鼠血小板衍生因子BB (PDGF-BB) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 PDGFB 靶点信息

PDGFB 分子靶点信息概述

分子名：PDGFB, platelet derived growth factor subunit B
别名：SSV
曾用名：SIS
全称：oncogene SIS; becaplermin; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor beta polypeptide

PDGFB 分子靶点综述

血小板衍生因子(PDGF)是众多调节细胞生长和分裂的因子之一。从化学结构上来说，PDGF是一种由两条A链(-AA)或两条B链(-BB)或AB链组合(-AB)形成的二聚体糖蛋白。特别是，它在血管生成、已存在的血管组织生长中起着重要作用。不受控制的血管生成是肿瘤的一个特征。肝细胞和不吸收的破骨细胞表达PDGF-BB，产生成骨细胞和骨头，血小板、巨噬细胞和肥大细胞也能产生PDGF-BB。在损伤部位，PDGF-BB促进中性粒细胞和巨噬细胞浸润，发挥清创作用，促进成纤维细胞分泌新的细胞外基质，促进IGF-1介导再上皮化。

小鼠 Mouse Pdgfb 分子靶点信息

分子名：Pdgfb, platelet derived growth factor, B polypeptide
别称：
- PDGF-B
- simian sarcoma oncogene
- Sis
基因序列：NCBI_Gene: 18591
蛋白序列：

小鼠 Mouse Pdgfb 靶点分子功能（预测）

Predicted to enable several functions, including platelet-derived growth factor binding activity; protein dimerization activity; and receptor ligand activity. Involved in several processes, including metanephric glomerular mesangium development; paracrine signaling; and positive regulation of glomerular filtration. Acts upstream of or within several processes, including animal organ development; nervous system development; and regulation of cell migration. Predicted to be located in several cellular components, including basolateral plasma membrane; dendrite; and neuronal cell body. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; cardiovascular system; extraembryonic component; female reproductive system; and nervous system. Human ortholog(s) of this gene implicated in basal ganglia calcification; clear cell renal cell carcinoma; connective tissue cancer (multiple); familial meningioma; and prostatic hypertrophy. Orthologous to human PDGFB (platelet derived growth factor subunit B).