EK2183

Mouse IL-12/IL-23p40 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：IL12, IL12B, NKSF, CLMF2
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.25 pg/mL
检测范围：4.69 pg/mL - 300 pg/mL
回收率：85% - 112%

在售SKU:70-EK2183/2-48, 70-EK2183/2-96

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库存号

描述

文章目录[隐藏]

分子信息
IL12B 分子靶点信息概述
IL12B 分子靶点综述
- 小鼠 Mouse Il12b 分子靶点信息
- 小鼠 Mouse Il12b 靶点分子功能（预测）

商品名	Mouse IL-12/IL-23p40 ELISA Kit (小鼠 IL-12/IL-23p40 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.3% - 3.0%；板间变异系数：3.6% - 6.2%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.25 pg/mL
检测范围	4.69 pg/mL - 300 pg/mL
回收率	85% - 112%
平均回收率	0.98
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-12/IL-23p40单克隆抗体的96孔聚苯乙烯酶标板小鼠IL-12/IL-23p40冻干标准品 IL-12/IL-23p40检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-12/IL-23p40抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-12/IL-23p40与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-12/IL-23p40的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-12/IL-23p40 ELISA Kit (小鼠 IL-12/IL-23p40 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 IL12B, NKSF, CLMF2 靶点信息

IL12B 分子靶点信息概述

分子名：IL12B, interleukin 12B
基因家族：Immunoglobulin like domain containing; Interleukins
别名：CLMF; IL-12B; NKSF; CLMF2
曾用名：NKSF2
全称：natural killer cell stimulatory factor-2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; natural killer cell stimulatory factor, 40 kD subunit; interleukin-12 beta chain; IL12, subunit p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

IL12B 分子靶点综述

白细胞介素12 (IL-12)由树突状细胞、巨噬细胞、中性粒细胞和人B淋巴母细胞株 (NC-37)在响应抗原刺激时自然产生。IL-12由4个а螺旋组成，是一个由2种独立基因--- IL-12A (p35)和IL-12B (p40)编码形成的异源二聚体细胞因子。蛋白合成之后可形成有生物活性的异源二聚体(p70)和同源二聚体p40。IL-12参与初始T细胞向Th1细胞分化的过程，作为T细胞刺激因子，它可刺激T细胞的生长及其功能发挥。IL-12具有抗血管生成活性，对自然杀伤细胞和T淋巴细胞活性有重要作用。同样地，IL-12与自身免疫相关。

小鼠 Mouse Il12b 分子靶点信息

分子名：Il12b, interleukin 12b
别称：
- IL-12 p40
- Il-12b
- Il-12p40
- IL-23 subunit p40
基因序列：NCBI_Gene: 16160
蛋白序列：
- UniprotKB: P43432
- UniprotKB: Q3ZAX5

小鼠 Mouse Il12b 靶点分子功能（预测）

Enables identical protein binding activity and interleukin-12 alpha subunit binding activity. Contributes to cytokine activity and cytokine receptor binding activity. Involved in positive regulation of T cell mediated cytotoxicity; positive regulation of T-helper 1 type immune response; and positive regulation of defense response to virus by host. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; defense response to other organism; and positive regulation of interferon-gamma production. Located in cell surface; cytoplasm; and extracellular space. Part of interleukin-12 complex and interleukin-23 complex. Is expressed in colon. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Orthologous to human IL12B (interleukin 12B).