EK2100

Mouse Growth Differentiation Factor 15 /GDF-15 ELISA Kit检测试剂盒（酶联免疫吸附法）

Name: Mouse Growth Differentiation Factor 15 /GDF-15 ELISA Kit检测试剂盒（酶联免疫吸附法）
SKU: EK2100
Availability: InStock

¥1,600.00 – ¥2,650.00

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Size
库存号

描述

背景介绍	细胞因子生长分化因子15 (GDF-15)是TGF - β超家族蛋白的BMP家族成员之一。GDF蛋白在胚胎发育中起着关键作用，特别是在骨骼、神经和肌肉系统。在小鼠中，GDF-15在多种激活的细胞类型中表达，包括肝细胞、巨噬细胞、雪旺细胞、心肌细胞、成骨细胞和脂肪细胞以及小肠和乳腺的上皮细胞。
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗鼠GDF-15抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的GDF-15与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中GDF-15的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
组分	包被羊抗小鼠GDF-15多克隆抗体的96孔聚苯乙烯酶标板小鼠GDF-15冻干标准品羊抗小鼠GDF-15检测抗体（偶联生物素）标准品稀释液（2份）检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜
分析类型	夹心ELISA
样本类型	血清、血浆，细胞培养上清及其他生物学样本
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
样本体积	100ul(稀释液)
灵敏度	0.72 pg/ml
标曲图
标准曲线范围	7.81 - 500 pg/ml
回收率范围	81.7 %-106.1 %
平均回收率	92%
板间变异系数	3.9 % - 8.1 %
板内变异系数	7.1 % - 8.9 %