EK287HS

Mouse CCL2/MCP-1 High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

  • 分子靶点:CCL2, MCP-1, MCAF, SMC-CF
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:2.08 pg/mL
  • 检测范围:23.44 pg/mL - 1500 pg/mL
  • 回收率:90% - 118%

在售SKU:70-EK287HS-48, 70-EK287HS-96

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描述

商品名

Mouse CCL2/MCP-1 High Sensitivity ELISA Kit (小鼠CCL2/MCP-1 高敏 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:3.1% - 4.9%;板间变异系数:3.0% - 4.5%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

2.08 pg/mL

检测范围

23.44 pg/mL - 1500 pg/mL

回收率

90% - 118%

平均回收率

1.07

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗CCL2/MCP-1单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠CCL2/MCP-1冻干标准品
  • CCL2/MCP-1检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠CCL2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的CCL2与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CCL2的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse CCL2/MCP-1 High Sensitivity ELISA Kit (小鼠CCL2/MCP-1 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 CCL2, MCP-1, MCAF, SMC-CF 靶点信息

CCL2 分子靶点信息概述

  • 分子名:CCL2, C-C motif chemokine ligand 2
  • 基因家族:Chemokine ligands
  • 别名:MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434
  • 曾用名:SCYA2
  • 全称:monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2

CCL2 分子靶点综述

趋化因子配体2(CCL2),又名单核细胞趋化蛋白1(MCP-1),是CC趋化因子家族的小分子量细胞因子。CCL2通过蛋白聚糖的粘多糖侧链锚定到内皮细胞的质膜上,主要由单核细胞、巨噬细胞和树突状细胞分泌。CCL2可招募单核细胞、记忆性T细胞和树突状细胞到组织损伤或感染引起的炎症位点。CCL2参与以单核细胞浸润为特征的一些疾病发病机制,如银屑病、类风湿性关节炎和动脉粥样硬化。CCL2参与发生在中枢神经系统的多种疾病的神经炎症过程,表现为神经元变性。

小鼠 Mouse Ccl2 分子靶点信息

小鼠 Mouse Ccl2 靶点分子功能(预测)

Enables CCR2 chemokine receptor binding activity and cytokine activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of leukocyte chemotaxis; and positive regulation of macromolecule metabolic process. Acts upstream of or within response to bacterium and response to wounding. Located in extracellular space. Is expressed in several structures, including heart; intestine smooth muscle circular layer; nervous system; retina; and vibrissa. Used to study age related macular degeneration. Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).

操作步骤


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