EK287HS

Mouse CCL2/MCP-1 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：CCL2, MCP-1, MCAF, SMC-CF
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：2.08 pg/mL
检测范围：23.44 pg/mL - 1500 pg/mL
回收率：90% - 118%

在售SKU:70-EK287HS-48, 70-EK287HS-96

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描述

文章目录[隐藏]

分子信息
CCL2 分子靶点信息概述
CCL2 分子靶点综述
- 小鼠 Mouse Ccl2 分子靶点信息
- 小鼠 Mouse Ccl2 靶点分子功能（预测）

商品名	Mouse CCL2/MCP-1 High Sensitivity ELISA Kit (小鼠CCL2/MCP-1 高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.1% - 4.9%；板间变异系数：3.0% - 4.5%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	2.08 pg/mL
检测范围	23.44 pg/mL - 1500 pg/mL
回收率	90% - 118%
平均回收率	1.07
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗CCL2/MCP-1单克隆抗体的96孔聚苯乙烯酶标板小鼠CCL2/MCP-1冻干标准品 CCL2/MCP-1检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素信号增强剂浓缩液信号增强剂稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠CCL2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的CCL2与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CCL2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse CCL2/MCP-1 High Sensitivity ELISA Kit (小鼠CCL2/MCP-1 高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 CCL2, MCP-1, MCAF, SMC-CF 靶点信息

CCL2 分子靶点信息概述

分子名：CCL2, C-C motif chemokine ligand 2
基因家族：Chemokine ligands
别名：MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434
曾用名：SCYA2
全称：monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2

CCL2 分子靶点综述

趋化因子配体2(CCL2)，又名单核细胞趋化蛋白1(MCP-1)，是CC趋化因子家族的小分子量细胞因子。CCL2通过蛋白聚糖的粘多糖侧链锚定到内皮细胞的质膜上，主要由单核细胞、巨噬细胞和树突状细胞分泌。CCL2可招募单核细胞、记忆性T细胞和树突状细胞到组织损伤或感染引起的炎症位点。CCL2参与以单核细胞浸润为特征的一些疾病发病机制，如银屑病、类风湿性关节炎和动脉粥样硬化。CCL2参与发生在中枢神经系统的多种疾病的神经炎症过程，表现为神经元变性。

小鼠 Mouse Ccl2 分子靶点信息

分子名：Ccl2, chemokine (C-C motif) ligand 2
别称：
- AI323594
- expressed sequence AI323594
- HC11
- MCAF
- MCP-1
- MCP1
- monocyte chemoattractant protein-1
- monocyte chemotactic protein
- Scya2
- Sigje
- small inducible cytokine A2
- small inducible gene JE
- SMC-CF
基因序列：NCBI_Gene: 20296
蛋白序列：

小鼠 Mouse Ccl2 靶点分子功能（预测）

Enables CCR2 chemokine receptor binding activity and cytokine activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of leukocyte chemotaxis; and positive regulation of macromolecule metabolic process. Acts upstream of or within response to bacterium and response to wounding. Located in extracellular space. Is expressed in several structures, including heart; intestine smooth muscle circular layer; nervous system; retina; and vibrissa. Used to study age related macular degeneration. Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).